Khan Shahid M, Kroeze Hans, Franke-Fayard Blandine, Janse Chris J
Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
Methods Mol Biol. 2013;923:139-50. doi: 10.1007/978-1-62703-026-7_9.
Genetically modified Plasmodium parasites are central gene function reagents in malaria research. The Rodent Malaria genetically modified DataBase (RMgmDB) ( www.pberghei.eu ) is a manually curated Web - based repository that contains information on genetically modified rodent malaria parasites. It provides easy and rapid access to information on the genotype and phenotype of genetically modified mutant and reporter parasites. Here, we provide guidelines for generating and describing rodent malaria parasite mutants. Standardization in describing mutant genotypes and phenotypes is important not only to enhance publication quality but also to facilitate cross-linking and mining data from multiple sources, and should permit information derived from mutant parasites to be used in integrative system biology approaches. We also provide guidelines on how to submit information to RMgmDB on non-published mutants, mutants that do not exhibit a clear phenotype, as well as negative attempts to disrupt/mutate genes. Such information helps to prevent unnecessary duplication of experiments in different laboratories, and can provide indirect evidence that these genes are essential for blood-stage development.
转基因疟原虫是疟疾研究中的核心基因功能试剂。啮齿动物疟疾转基因数据库（RMgmDB）（www.pberghei.eu）是一个基于网络的人工管理知识库，其中包含转基因啮齿动物疟原虫的相关信息。它能方便快捷地获取转基因突变体和报告基因寄生虫的基因型和表型信息。在此，我们提供生成和描述啮齿动物疟原虫突变体的指南。描述突变体基因型和表型的标准化不仅对提高出版物质量很重要，而且有助于跨来源的数据交叉链接和挖掘，并且应允许将来自突变寄生虫的信息用于综合系统生物学方法。我们还提供了关于如何向RMgmDB提交未发表突变体、未表现出明确表型的突变体以及破坏/突变基因的阴性尝试的信息。这些信息有助于防止不同实验室进行不必要的重复实验，并可提供间接证据表明这些基因对血期发育至关重要。
