利用RNA干扰技术使冈比亚按蚊中的基因和等位基因沉默。

Silencing of genes and alleles by RNAi in Anopheles gambiae.

作者信息

Lamacchia Marina, Clayton John R, Wang-Sattler Rui, Steinmetz Lars M, Levashina Elena A, Blandin Stéphanie A

机构信息

Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France.

出版信息

Methods Mol Biol. 2013;923:161-76. doi: 10.1007/978-1-62703-026-7_11.

DOI:10.1007/978-1-62703-026-7_11

PMID:22990777

Abstract

Anopheles gambiae mosquitoes are the major vectors of human malaria parasites. However, mosquitoes are not passive hosts for parasites, actively limiting their development in vivo. Our current understanding of the mosquito antiparasitic response is mostly based on the phenotypic analysis of gene knockdowns obtained by RNA interference (RNAi), through the injection or transfection of long dsRNAs in adult mosquitoes or cultured cells, respectively. Recently, RNAi has been extended to silence specifically one allele of a given gene in a heterozygous context, thus allowing to compare the contribution of different alleles to a phenotype in the same genetic background.

摘要

冈比亚按蚊是人类疟原虫的主要传播媒介。然而，蚊子并非寄生虫的被动宿主，它们会主动限制寄生虫在体内的发育。我们目前对蚊子抗寄生虫反应的理解主要基于通过RNA干扰（RNAi）获得的基因敲低的表型分析，分别是通过向成年蚊子或培养细胞中注射或转染长双链RNA（dsRNA）。最近，RNAi已扩展到在杂合背景下特异性沉默给定基因的一个等位基因，从而能够比较不同等位基因在相同遗传背景下对表型的贡献。