Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore-560 012, India.
Dalton Trans. 2012 Nov 21;41(43):13330-7. doi: 10.1039/c2dt31335a.
Two new hydroxynaphthyl-hydrazone based fluorogenic chemosensors R1 and R2 have been synthesized by Schiff base condensation of Tris(4-formylphenyl)amine with 1-hydroxynaphthalene-2-hydrazide and 1-hydroxynaphthalene-2-carbohydrazone, respectively. They are examined as highly selective and sensitive receptors for Cu2+ ions in aqueous medium. Electronic absorption as well as fluorescence titration studies of receptors R1 and R2 with different metal cations in H2O/CH3CN medium showed highly selective and very rapid (<2 min) binding affinity towards Cu2+ ions even in the presence of other commonly coexisting metal ions such as Na+, K+, Mg2+, Ca2+, Mn2+, Fe2+, Co2+, Ni2+, Zn2+, Cd2+ and Hg2+. Quantification of the fluorescence titration analysis indicated that these newly synthesized receptors (R1 and R2) can indicate the presence of Cu2+ ions even at very low concentrations of 598 and 676 ppt, respectively. In addition, the propensity of these receptors as bio-imaging fluorescent probes to detect Cu2+ ions in human cervical HeLa cancer cell lines and their cytotoxicity against HeLa cells have been investigated.
两种新的基于羟萘基腙的荧光化学传感器 R1 和 R2 通过三(4-醛基苯基)胺与 1-羟基萘-2-腙和 1-羟基萘-2-甲酰腙的席夫碱缩合合成。它们被用作在水介质中检测 Cu2+离子的高选择性和高灵敏度受体。受体 R1 和 R2 与不同金属阳离子在 H2O/CH3CN 介质中的电子吸收和荧光滴定研究表明，即使在存在其他常见共存金属离子（如 Na+、K+、Mg2+、Ca2+、Mn2+、Fe2+、Co2+、Ni2+、Zn2+、Cd2+和 Hg2+）的情况下，它们对 Cu2+离子具有高度选择性和非常快速（<2 分钟）的结合亲和力。荧光滴定分析的定量表明，这些新合成的受体（R1 和 R2）甚至可以在非常低的浓度（分别为 598 和 676 ppt）下指示 Cu2+离子的存在。此外，还研究了这些受体作为生物成像荧光探针检测人宫颈 HeLa 癌细胞系中 Cu2+离子的倾向及其对 HeLa 细胞的细胞毒性。
