青少年5-羟色胺转运体基因多态性与创伤后应激障碍的关联研究

[Association study of the 5-HTT polymorphism and posttraumatic stress disorder in adolescents].

作者信息

Zhao Lian-Sheng, Wang Ying-Cheng, Yu Yue, Li Tao, Ma Xiao-Hong

机构信息

The Psychiatric Laboratory, West China Hospital, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2012 Jul;43(4):558-61.

PMID:22997896

Abstract

OBJECTIVE

To study the association between 5-HTTVNTR polymorphism and posttraumatic stress disorder (PTSD) in Chinese Han adolescents after the Wenchuan Earthquake, and investigation of the genetic mechanism of PTSD.

METHODS

Polymerase chain reaction (PCR) was used to detect the distributive frequency of 5-HTTVNTR polymorphism of 236 PTSD patients (patient group) and 234 normal people (control group), Plink version 1.07 was used to analyze the genotyping results.

RESULTS

The frequency of 5-HTTVNTR allele 12 in PTSD group was significantly higher than control group (93.2% vs. 88.9%, chi2 = 5.42, P = 0.020). The 12/12 genotype in PTSD group and control group was 88.1% and 79.7%; 10/12 genotype was 10.2% and 19.6%; 10/10 genotype was 1.7% and 1.7%. The distributive frequency of all three genotypes (12/12, 10/12, 10/10) showed statistically significant (P = 0.023).

CONCLUSION

The allele 12 of 5-HTTVNTR may increase the risk of PTSD in Chinese Han adolescents, 12/12 genotype may be the susceptibility gene, and heterozygote 10/12 may act as the protective factor of PTSD.

摘要

目的

研究5-羟色胺转运体基因可变数目串联重复序列（5-HTTVNTR）多态性与汶川地震后中国汉族青少年创伤后应激障碍（PTSD）的关系，并探讨PTSD的遗传机制。

方法

采用聚合酶链反应（PCR）检测236例PTSD患者（患者组）和234例正常人（对照组）5-HTTVNTR多态性的分布频率，使用Plink 1.07软件分析基因分型结果。

结果

PTSD组5-HTTVNTR等位基因12的频率显著高于对照组（93.2%对88.9%，χ² = 5.42，P = 0.020）。PTSD组和对照组的12/12基因型分别为88.1%和79.7%；10/12基因型分别为10.2%和19.6%；10/10基因型分别为1.7%和1.7%。三种基因型（12/12、10/12、10/10）的分布频率差异有统计学意义（P = 0.023）。