Tu Tzong-Yang
Department of Otolaryngology, Taipei Veterans General Hospital, and Department of Otolaryngology, National Yang Ming University School of Medicine, Taipei, Taiwan, ROC.
Audiol Neurootol. 2013;18(1):9-16. doi: 10.1159/000342688. Epub 2012 Sep 21.
This study evaluated the long-term histologic changes of grafted auricular cartilage in rabbit tympanic bullae.
Auricular cartilage with or without perichondrium was prepared and cut into small pieces to obliterate the rabbit tympanic bullae. The histologic changes of the grafted cartilage in both groups were compared 20 months after surgery.
Remarkable spongy bony trabeculae of mature lamellar bone with red bone marrow formation were observed in the perichondrium-preserved group. Parts of the grafted cartilage pieces were invaded and replaced by bone and bone marrow. The grafted cartilage pieces grossly maintained their original polygonal shapes, and no osteochondral tissue regeneration was observed in the perichondrium-removed group. The viable chondrocyte ratios were 46.21 ± 5.58 versus 27.80 ± 4.81%, and the minimal resorption ratios were 10.31 ± 3.27 versus 2.98 ± 1.48% in the perichondrium-preserved (n = 14) and -removed groups (n = 12, p < 0.05). The tissue ratios were cartilage: 38.18 ± 8.76 versus 52.97 ± 9.30%; lamellar bone: 18.49 ± 5.31 versus 0.82 ± 0.43%; bone marrow: 20.72 ± 6.27 versus 0.00 ± 0.00%; fibrous tissue: 10.13 ± 2.74 versus 5.81 ± 2.20%, and adipose tissue: 12.01 ± 4.48 versus 40.70 ± 7.83% in the perichondrium-preserved and -removed groups. The differences were all statistically significant (p < 0.05).
A space-filling mass effect with minimal resorption of the cartilage pieces was observed in the perichondrium-removed group. In addition to this mass effect, the progenitor cells in the preserved perichondrium allowed active bone tissue regeneration and cartilage resorption in the perichondrium-preserved group.
本研究评估了兔鼓室内移植耳软骨的长期组织学变化。
制备带或不带软骨膜的耳软骨并切成小块,用于填充兔鼓室。术后20个月比较两组移植软骨的组织学变化。
在保留软骨膜组中观察到成熟板层骨显著的海绵状骨小梁并伴有红骨髓形成。部分移植软骨块被骨和骨髓侵入并替代。移植软骨块大体上保持其原始多边形形状,在去除软骨膜组中未观察到骨软骨组织再生。保留软骨膜组(n = 14)和去除软骨膜组(n = 12,p < 0.05)的活软骨细胞比率分别为46.21 ± 5.58% 和27.80 ± 4.81%,最小吸收比率分别为10.31 ± 3.27% 和2.98 ± 1.48%。保留软骨膜组和去除软骨膜组的组织比率分别为:软骨:38.18 ± 8.76% 对52.97 ± 9.30%;板层骨:18.49 ± 5.31% 对0.82 ± 0.43%;骨髓:20.72 ± 6.27% 对0.00 ± 0.00%;纤维组织:10.13 ± 2.74% 对5.81 ± 2.20%,脂肪组织:12.01 ± 4.48% 对40.70 ± 7.83%。差异均具有统计学意义(p < 0.05)。
在去除软骨膜组中观察到软骨块吸收最少的占位效应。除了这种占位效应外,保留的软骨膜中的祖细胞使保留软骨膜组中出现活跃的骨组织再生和软骨吸收。