Department of Basic Pathology, National Defense Medical College, Tokorozawa, Saitama, Japan.
APMIS. 2012 Nov;120(11):890-900. doi: 10.1111/j.1600-0463.2012.02919.x. Epub 2012 May 24.
We examined the potential role of cell-cycle dysregulation in the development and histological progression of adult testicular germ cell tumors (TGCTs). Expressions of p27(Kip1) -interacting cell-cycle regulators (down-regulation of p27(Kip1) and overexpression of Skp2, Cks1, cyclin A, and cyclin E) and Ki-67 labeling index (LI) were immunohistochemically examined in histological components of 50 intratubular germ cell neoplasms, unclassified (IGCNUs); 74 seminomas; and 25 embryonal carcinomas, identified from 88 patients. Altered expression of p27(Kip1) , Skp2, Cks1, cyclin A, and cyclin E was observed in 20%, 12%, 16%, 10%, and 24% of IGCNUs; 26%, 36%, 27%, 89%, and 23% of seminomas; and 48%, 68%, 56%, 100%, and 60% of embryonal carcinomas, respectively. A significant difference in the frequency of Skp2 and cyclin A overexpression was observed between IGCNUs and seminomas. Significantly more frequent alterations of Skp2, Cks1, and cyclin E and p27(Kip1) were detected in embryonal carcinomas than in seminomas. Alterations of all cell-cycle regulators were significantly more frequent in embryonal carcinomas than in IGCNUs. The mean Ki-67 LI significantly increased from IGCNU (21.2%) through seminoma (34.7%) to embryonal carcinoma (54.2%). These results suggest that alterations of the p27(Kip1) -interacting cell-cycle regulators are common in TGCTs and may be involved in their histological progression.
我们研究了细胞周期失调在成人睾丸生殖细胞肿瘤（TGCTs）的发展和组织学进展中的潜在作用。在 88 例患者中确定的 50 例管内生殖细胞肿瘤未分类（IGCNUs）；74 例精原细胞瘤；和 25 例胚胎癌的组织学成分中，用免疫组织化学方法检查了 p27（Kip1）相互作用的细胞周期调节剂（p27（Kip1）下调和 Skp2、Cks1、细胞周期蛋白 A 和细胞周期蛋白 E 过表达）和 Ki-67 标记指数（LI）的表达。在 IGCNUs 中观察到 p27（Kip1）、Skp2、Cks1、细胞周期蛋白 A 和细胞周期蛋白 E 的表达改变分别为 20%、12%、16%、10%和 24%；精原细胞瘤分别为 26%、36%、27%、89%和 23%；胚胎癌分别为 48%、68%、56%、100%和 60%。IGCNUs 和精原细胞瘤之间 Skp2 和细胞周期蛋白 A 过表达的频率存在显著差异。在胚胎癌中，Skp2、Cks1 和细胞周期蛋白 E 以及 p27（Kip1）的改变更为频繁。与精原细胞瘤相比，胚胎癌中 Skp2、Cks1 和细胞周期蛋白 E 的改变更为频繁。所有细胞周期调节剂的改变在胚胎癌中比在 IGCNUs 中更为频繁。Ki-67 LI 均值从 IGCNU（21.2%）到精原细胞瘤（34.7%）再到胚胎癌（54.2%）显著增加。这些结果表明，p27（Kip1）相互作用的细胞周期调节剂的改变在 TGCTs 中很常见，可能参与其组织学进展。
