Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, Saltillo, Coahuila, Mexico.
Colloids Surf B Biointerfaces. 2013 Jan 1;101:392-7. doi: 10.1016/j.colsurfb.2012.07.023. Epub 2012 Jul 25.
Tannase from Aspergillus niger was partitioned in aqueous two-phase systems composed by polyethyleneglycol of molar mass 400, 600 and 1000 and potassium phosphate. Tannase was found to be partitioned toward the salt-rich phase in all systems, with partition coefficients lower than 0.5. Partition coefficients values and low entropic and enthalpic changes associated with tannase partition suggest that the entropic effect may be the driving force of the concentration of the enzyme in the bottom phase due to the high molar mass of the enzyme. The process was significantly influenced by the top phase/bottom phase volume ratio. When the fungal culture broth was partitioned in these systems, a good performance was found, since the enzyme recovery in the bottom phase of the system composed by polyethyleneglycol 1000 was around 96% with a 7.0-fold increase in purity.
黑曲霉单宁酶在由相对分子质量为 400、600 和 1000 的聚乙二醇和磷酸钾组成的双水相系统中进行分配。结果表明，单宁酶分配到所有系统的盐富相，分配系数均低于 0.5。分配系数值以及与单宁酶分配相关的低熵和焓变表明，由于酶的高相对分子质量，熵效应可能是酶在底相中浓缩的驱动力。该过程受顶相/底相体积比的显著影响。当真菌培养液在这些系统中进行分配时，发现了良好的效果，因为在由聚乙二醇 1000 组成的系统的底相中，酶的回收率约为 96%，纯度提高了 7 倍。
