载染料脂质体在人乳腺癌敏感细胞和耐药细胞中蓄积情况的比较研究。
Comparative study of dye-loaded liposome accumulation in sensitive and resistant human breast cancer cells.
作者信息
Yefimova S L, Kurilchenko I Yu, Tkacheva T N, Rozhkov V A, Sorokin A V, Lukianova N Yu, Bezdenezhnykh N A, Malyukin Yu V, Chekhun V F
机构信息
Institute for Scintillation Materials, NAS of Ukraine, Kharkov 61001, Ukraine.
出版信息
Exp Oncol. 2012 Jul;34(2):101-6.
UNLABELLED
The aim of this research is to study the dynamics and efficiency of liposome accumulation in sensitive and resistant human breast cancer cells.
METHODS
Methods of fluorescence microscopy, fluorescence microspectroscopy and MTT-test have been used.
RESULTS
The liposome-to-cell interaction and dye cellular uptake in sensitive, cisplatin-resistant and doxorubicin-resistant MCF-7 human breast cancer cells have been analyzed using time changes in both fluorescence resonance energy transfer signal from the donor probe DiO to the acceptor one DiI preloaded in liposomes and cell image brightness.
CONCLUSION
Obtained results show that resistant cells accumulate dye-loaded liposomes more effectively and reveal more effective dye molecule cellular uptake.
未标记
本研究的目的是研究脂质体在敏感和耐药人乳腺癌细胞中积累的动力学和效率。
方法
采用荧光显微镜、荧光显微光谱法和MTT试验方法。
结果
利用脂质体中预加载的供体探针DiO到受体探针DiI的荧光共振能量转移信号的时间变化以及细胞图像亮度，分析了敏感、顺铂耐药和阿霉素耐药的MCF-7人乳腺癌细胞中脂质体与细胞的相互作用以及染料的细胞摄取。
结论
获得的结果表明，耐药细胞更有效地积累载有染料的脂质体，并显示出更有效的染料分子细胞摄取。
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