利用 CD 和 FTIR 光谱研究大豆（Glycine max L.）种子 LEA 蛋白 GmPM6、GmPM11 和 GmPM30 的功能。
Functional studies of soybean (Glycine max L.) seed LEA proteins GmPM6, GmPM11, and GmPM30 by CD and FTIR spectroscopy.
机构信息
Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
出版信息
Plant Sci. 2012 Nov;196:152-9. doi: 10.1016/j.plantsci.2012.07.012. Epub 2012 Aug 20.
The protein and mRNA levels of late embryogenesis abundant (LEA) genes may be linked to osmotic stresses. Here, we characterized three soybean hydrophilic LEA proteins--GmPM11 (LEA I), GmPM6 (LEA II), and GmPM30 (LEA III)--by circular dichroism and Fourier transform infrared spectroscopy. Structural analysis revealed that the LEA proteins adopted high amounts of disordered conformations in solution and underwent conformational changes with hydrophobicity and desiccation induction. Macromolecular interaction studies revealed that the GmPM proteins interact with non-reducing sugars and phospholipids. GmPM6 and GmPM30 but not GmPM11 could prevent beta-aggregation of poly-L-lysine after slow drying. We discuss the possible functions of hydrophilic LEA proteins in maturing seeds.
晚期胚胎丰富蛋白（LEA）的基因的蛋白质和 mRNA 水平可能与渗透胁迫有关。在这里，我们通过圆二色性和傅里叶变换红外光谱对三种大豆亲水 LEA 蛋白 - GmPM11（LEA I）、GmPM6（LEA II）和 GmPM30（LEA III）进行了表征。结构分析表明，LEA 蛋白在溶液中采用大量无序构象，并随着疏水性和干燥诱导发生构象变化。大分子相互作用研究表明，GmPM 蛋白与非还原糖和磷脂相互作用。GmPM6 和 GmPM30 但不是 GmPM11 可以在缓慢干燥后防止聚-L-赖氨酸的β-聚集。我们讨论了亲水 LEA 蛋白在成熟种子中的可能功能。
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