来自马豆（单花大翼豆）的耐汞氯化物根瘤菌的质粒图谱。
Plasmid profiles of mercuric chloride tolerant rhizobia from horse gram (Macrotyloma uniflorum).
作者信息
Edulamudi Prabhavati, Johnson Antony A M, Divi Venkata Ramana Sai Gopal, Konada Veera Mallaiah
机构信息
Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur- 522 510, India.
出版信息
J Environ Biol. 2012 Mar;33(2):187-90.
Thirty two rhizobia were isolated from the fresh healthy root nodules of horse gram. They were found to be highly salt tolerant. They were identified as rhizobia by cultural, biochemical and 16S rRNA sequence. The sequences of the four selected isolates were deposited in the NCBI GenBank. The obtained accession numbers were GQ483457, GQ483458, GQ483459 and GQ483460. All the rhizobia were able to grow at 10 ppm mercuric chloride concentration. Four isolates HGR-11, 16, 30 and 31 were used to study the effect of different concentrations of mercuric chloride on the growth of rhizobia. These isolates were able to grow at 30 ppm concentration also. In these isolates, HGR-11 and HGR-30 showed maximum growth at 20 ppm than at control. These isolates contained one mega plasmid (-22 kb) at 20 ppm mercuric chloride concentration.
从新鲜健康的绿豆根瘤中分离出32株根瘤菌。发现它们具有高度耐盐性。通过培养、生化和16S rRNA序列鉴定为根瘤菌。所选4株分离菌的序列保存在NCBI基因库中。获得的登录号为GQ483457、GQ483458、GQ483459和GQ483460。所有根瘤菌都能在10 ppm氯化汞浓度下生长。使用4株分离菌HGR - 11、16、30和31研究不同浓度氯化汞对根瘤菌生长的影响。这些分离菌在30 ppm浓度下也能生长。在这些分离菌中，HGR - 11和HGR - 30在20 ppm时比对照表现出最大生长。在20 ppm氯化汞浓度下，这些分离菌含有一个大质粒（-22 kb）。
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