一种具有与毒蕈碱受体类似的抗抑郁结合特性的单克隆抗丙咪嗪抗体。

A monoclonal anti-imipramine antibody with antidepressant binding properties similar to the muscarinic receptor.

作者信息

Ronayne L, McInerney M, Phillips O M, Regan C M, Williams D C

机构信息

Department of Biochemistry, Trinity College, Dublin, Ireland.

出版信息

Biochem Pharmacol. 1990 Feb 1;39(3):507-11. doi: 10.1016/0006-2952(90)90057-r.

DOI:10.1016/0006-2952(90)90057-r

PMID:2306263

Abstract

A murine monoclonal antibody raised using imipramine conjugated to bovine serum albumin bound free imipramine with high affinity (KD = 24 nM). The antibody had a binding affinity profile for tricyclic and other uptake-inhibitor type antidepressants which correlated highly with the affinities of the same compounds for the brain acetylcholine muscarinic receptor, but not for plasma-membrane 5-hydroxytryptamine carriers. This antibody was not similar in binding properties to polyclonal antisera produced using the same antigen but was highly similar in binding properties to a monoclonal anti-nortriptyline antibody, ANT3, raised by Marulo et al. (Marulo S, Hoebeke J, Guillet JG, Andre C and Strosberg AD, J Immunol 138: 524-526, 1987) using a different antigen.

摘要

一种用与牛血清白蛋白偶联的丙咪嗪制备的鼠单克隆抗体，能以高亲和力（KD = 24 nM）结合游离丙咪嗪。该抗体对三环类及其他摄取抑制剂型抗抑郁药的结合亲和力谱，与相同化合物对脑乙酰胆碱毒蕈碱受体的亲和力高度相关，但与质膜5-羟色胺载体的亲和力无关。该抗体的结合特性与使用相同抗原产生的多克隆抗血清不同，但与Marulo等人（Marulo S、Hoebeke J、Guillet JG、Andre C和Strosberg AD，《免疫学杂志》138：524 - 526，1987）使用不同抗原制备的抗去甲替林单克隆抗体ANT3的结合特性高度相似。