甲拌磷对赤子爱胜蚓体腔细胞溶酶体膜稳定性的对映选择性影响。
Enantioselective effects of methamidophos on the coelomocytes lysosomal membrane stability of Eisenia fetida.
机构信息
Research Center of Environmental Science, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.
出版信息
Bull Environ Contam Toxicol. 2012 Dec;89(6):1161-4. doi: 10.1007/s00128-012-0861-y. Epub 2012 Oct 17.
Many of organophosphorous insecticides are chiral compounds. In this study, the enantioselective effects of organophosphate insecticide methamidophos on the coelomocytes lysosomal membrane stability of earthworm Eisenia fetida were studied: (1) The enantiomers of methamidophos were absolutely separated by high-performance liquid chromatography with a commercial chiral column; (2) The neutral red retention assay was used to judge the lysosomal membrane stability. The results showed that with the concentration increasing, lysosomal membranes have been significantly destroyed by individual stereoisomers and racemate of methamidophos. The neutral red retention times were significantly descended from 76.88 to 29.78 min. Both (+)- and (-)-methamidophos showed more prone to destroy the integrity of the lysosomal membrane than the racemate. However, the different effect between stereoisomers is slight.
许多有机磷杀虫剂都是手性化合物。本研究采用高效液相色谱法（HPLC），利用商品化的手性柱对甲胺磷的对映体进行绝对拆分，研究了有机磷杀虫剂甲胺磷对赤子爱胜蚓（Eisenia fetida）体腔细胞溶酶体膜稳定性的对映选择性影响：（1）利用高效液相色谱法（HPLC），利用商品化的手性柱对甲胺磷的对映体进行绝对拆分；（2）采用中性红保留试验判断溶酶体膜的稳定性。结果表明，随着浓度的增加，甲胺磷的各个对映体和外消旋体对溶酶体膜的破坏作用明显，中性红的保留时间从 76.88 分钟显著下降到 29.78 分钟。（+）-和（-）-甲胺磷比外消旋体更容易破坏溶酶体膜的完整性，但两种对映体之间的差异很小。
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