Ion flux measurements using the MIFE technique.

Noninvasive microelectrode ion flux measurements (the MIFE™ technique) allow the concurrent quantification of net fluxes of several ions with high spatial (several μm) and temporal (ca 5 s) resolution. The MIFE technique has become a popular tool for studying the adaptive responses of plant cells and tissues to a large number of abiotic and biotic stresses. This chapter briefly summarizes some key findings on spatial and temporal organization of plant nutrient acquisition obtained by the MIFE technique, as well as the MIFE contribution towards elucidating the mechanisms behind a plant's perception and signaling of major abiotic stresses. The full protocols for microelectrode fabrication, calibration, and use are then given, and two basic routines for mapping root ion flux profiles and studying transient ion flux kinetics are given.