Clinic of Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine (Vetmeduni) Vienna, Vienna 1210, Austria.
Vet Rec. 2012 Dec 8;171(23):594. doi: 10.1136/vr.101107. Epub 2012 Oct 23.
The objective of this study was to compare the inflammatory response within the abdominal cavity between three surgical methods. The study comprised 45 cows with left displacement of the abomasum, which were allocated into three groups (n = 15). Right flank laparotomy and omentopexy (group R), left flank laparotomy and omentopexy (group L), and laparoscopic abomasopexy (group J) have been applied. Laparoscopic abomasopexy was the only technique that requires perforation of the abomasal wall. Blood and peritoneal fluid (PF) samples were obtained before, and on days 1, 2 and 3 after surgery. Macroscopic and microscopic evaluation of PF were performed. Cytological and biochemical parameters were analysed in blood and PF. No bacteria were present in PF after surgery. The number of PF leukocytes increased in all groups on day 1 after surgery with the highest value after laparoscopy (median, 1st quartile, 3rd quartile, R: 13.1, 6.4, 16.0; L: 13.6, 9.9, 17.4; J: 33.7, 21.1, 46.9 G/l). Laparotomy resulted in an increase of blood and PF CK on day 1 after surgery, whereas, laparoscopy caused an increased PF CK only. All groups had elevated PF D-dimer concentrations before surgery, with further increase in groups R and L on day 1 after surgery.
本研究旨在比较三种手术方法在腹腔内的炎症反应。研究纳入了 45 头左侧真胃变位奶牛,将其分为三组(每组 15 头)。分别进行右侧经腹侧剖腹术和大网膜固定术(R 组)、左侧经腹侧剖腹术和大网膜固定术(L 组)和腹腔镜真胃固定术(J 组)。腹腔镜真胃固定术是唯一需要刺穿真胃壁的技术。在手术前、手术后第 1、2 和 3 天采集血液和腹腔液(PF)样本。对 PF 进行宏观和微观评估。分析血液和 PF 中的细胞学和生化参数。手术后 PF 中未检出细菌。所有组在手术后第 1 天 PF 白细胞数量均增加,腹腔镜组最高(中位数,1 四分位数,3 四分位数,R:13.1,6.4,16.0;L:13.6,9.9,17.4;J:33.7,21.1,46.9 G/L)。剖腹术导致手术后第 1 天血液和 PF CK 增加,而腹腔镜仅导致 PF CK 增加。所有组在手术前 PF D-二聚体浓度升高,R 组和 L 组在手术后第 1 天进一步升高。