Chauhan Vinita, Howland Matthew
Consumer and Clinical Radiation Protection Bureau, Health Canada, Healthy Environment and Consumer Safety Branch, Ottawa, ON, Canada K1A 0K9. vinita
ScientificWorldJournal. 2012;2012:205038. doi: 10.1100/2012/205038. Epub 2012 Oct 11.
This study examined alpha (α-) particle radiation effects on global changes in gene expression in human leukemic monocytic cells (THP-1) for the purposes of mining for candidate biomarkers that could be used for the development of a biological assessment tool. THP-1 cells were exposed to α-particle radiation at a dose range of 0 to 1.5 Gy. Twenty-four hours and three days after exposure gene expression was monitored using microarray technology. A total of 16 genes were dose responsive and classified as early onset due to their expression 24 h after exposure. Forty-eight transcripts were dose responsive and classified as late-onset as they were expressed 72 h after exposure. Among these genes, 6 genes were time and dose responsive and validated further using alternate technology. These transcripts were upregulated and associated with biological processes related to immune function, organelle stability and cell signalling/communication. This panel of genes merits further validation to determine if they are strong candidate biomarkers indicative of α-particle exposure.
本研究检测了α粒子辐射对人白血病单核细胞（THP-1）基因表达整体变化的影响，目的是挖掘可用于开发生物评估工具的候选生物标志物。将THP-1细胞暴露于0至1.5 Gy剂量范围的α粒子辐射下。暴露后24小时和三天，使用微阵列技术监测基因表达。共有16个基因呈剂量反应性，因其在暴露后24小时表达而被归类为早期发作。48个转录本呈剂量反应性，因其在暴露后72小时表达而被归类为晚期发作。在这些基因中，6个基因呈时间和剂量反应性，并使用替代技术进一步验证。这些转录本上调，并与免疫功能、细胞器稳定性和细胞信号传导/通讯相关的生物学过程有关。这组基因值得进一步验证，以确定它们是否是指示α粒子暴露的强有力候选生物标志物。
