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暴露于α粒子辐射的人白血病单核细胞系(THP-1)的基因组分析。

Genomic profiling of a human leukemic monocytic cell-line (THP-1) exposed to alpha particle radiation.

作者信息

Chauhan Vinita, Howland Matthew

机构信息

Consumer and Clinical Radiation Protection Bureau, Health Canada, Healthy Environment and Consumer Safety Branch, Ottawa, ON, Canada K1A 0K9. vinita

出版信息

ScientificWorldJournal. 2012;2012:205038. doi: 10.1100/2012/205038. Epub 2012 Oct 11.

Abstract

This study examined alpha (α-) particle radiation effects on global changes in gene expression in human leukemic monocytic cells (THP-1) for the purposes of mining for candidate biomarkers that could be used for the development of a biological assessment tool. THP-1 cells were exposed to α-particle radiation at a dose range of 0 to 1.5 Gy. Twenty-four hours and three days after exposure gene expression was monitored using microarray technology. A total of 16 genes were dose responsive and classified as early onset due to their expression 24 h after exposure. Forty-eight transcripts were dose responsive and classified as late-onset as they were expressed 72 h after exposure. Among these genes, 6 genes were time and dose responsive and validated further using alternate technology. These transcripts were upregulated and associated with biological processes related to immune function, organelle stability and cell signalling/communication. This panel of genes merits further validation to determine if they are strong candidate biomarkers indicative of α-particle exposure.

摘要

本研究检测了α粒子辐射对人白血病单核细胞(THP-1)基因表达整体变化的影响,目的是挖掘可用于开发生物评估工具的候选生物标志物。将THP-1细胞暴露于0至1.5 Gy剂量范围的α粒子辐射下。暴露后24小时和三天,使用微阵列技术监测基因表达。共有16个基因呈剂量反应性,因其在暴露后24小时表达而被归类为早期发作。48个转录本呈剂量反应性,因其在暴露后72小时表达而被归类为晚期发作。在这些基因中,6个基因呈时间和剂量反应性,并使用替代技术进一步验证。这些转录本上调,并与免疫功能、细胞器稳定性和细胞信号传导/通讯相关的生物学过程有关。这组基因值得进一步验证,以确定它们是否是指示α粒子暴露的强有力候选生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e50e/3477555/e1d248c1b8e8/TSWJ2012-205038.001.jpg

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