来自锡金沙棘根瘤中的弗兰克氏菌的分子多样性。
Molecular diversity of Frankia from root nodules of Hippophae salicifolia D.Don found in Sikkim.
机构信息
Molecular Genetics Laboratory, Department of Botany, North Eastern Hill University, Shillong, 793 022 India.
出版信息
Indian J Microbiol. 2009 Jun;49(2):196-200. doi: 10.1007/s12088-009-0025-x. Epub 2009 Apr 24.
Abstract
Molecular diversity of Frankia was assessed directly from the root nodules of Hippophae salicifolia naturally occurring in North Sikkim. Amplicon restriction patterns (ARPs) were developed by digesting 16S-ITS-23S amplicons with RsaI. Three ARPs were detected, showing diversity among strains of Frankia that nodulate Hippophae. This was confirmed by sequencing one amplicon each for the three ARPs. Therefore, ARP can be used as a tool for screening amplicons for nucleotide sequencing.
摘要
直接从北锡金自然生长的沙棘根瘤中评估弗兰克氏菌的分子多样性。通过用 RsaI 消化 16S-ITS-23S 扩增子来开发扩增子限制图谱 (ARPs)。检测到三种 ARPs，显示出弗兰克氏菌菌株之间的多样性，这些菌株能够为沙棘结瘤。这通过对三种 ARP 中的每一种进行一个扩增子的测序得到证实。因此，ARPs 可以用作筛选用于核苷酸测序的扩增子的工具。
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