结核分枝杆菌H(37)R(a)分级排泄分泌（EST-DE(1)）抗原在肺结核中的诊断潜力
Diagnostic potential of fractionatedMycobacterium tuberculosis H(37)R(a) excretory-secretory (EST-DE(1)) antigen in pulmonary tuberculosis.
作者信息
Lodam A N, Pramanik J, Reddy M V, Narang P, Harinath B C
机构信息
J.B. Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, 442 102 Sevagram, Wardha India.
出版信息
Indian J Clin Biochem. 1997 Dec;12(1):71-7. doi: 10.1007/BF02867960.
Tuberculosis is emerging as a major public health problem in developing and developed world. Early and precise diagnosis is of prime importance in successful control of infection. Indirect ELISA with penicillinase as marker was developed using purifiedM. tuberculosis excretory-secretory (EST-DE(1)) antigen for detecting IgG antibodies in pulmonary tuberculosis. The assay System gave a overall sensitivity of 82% for both smear positive and smear negative pulmonary tuberculosis cases with a specificity of 84%. The positive and negative predictive values were 75% and 88% respectivaly. Further studies with EST-DE(1) antigen revealed that, it contains two of the active antigen fractions of Mtb EST antigen i.e. Mtb EST-4 (56-68 KDa) and Mtb EST-6 (37-45 KDa), as demonstrated by inhibition ELISA. Reactivity with monoclonal antibodies HGT 3a showed the presence of 38 KDa molecule in EST-DE(1) antigen.
结核病正在成为发展中国家和发达国家的一个主要公共卫生问题。早期准确诊断对于成功控制感染至关重要。利用纯化的结核分枝杆菌排泄分泌（EST-DE(1)）抗原开发了以青霉素酶为标志物的间接ELISA法，用于检测肺结核患者的IgG抗体。该检测系统对涂片阳性和涂片阴性肺结核病例的总体敏感性为82%，特异性为84%。阳性和阴性预测值分别为75%和88%。对EST-DE(1)抗原的进一步研究表明，通过抑制ELISA证实，它包含结核分枝杆菌EST抗原的两个活性抗原组分，即结核分枝杆菌EST-4（56-68 kDa）和结核分枝杆菌EST-6（37-45 kDa）。与单克隆抗体HGT 3a的反应表明EST-DE(1)抗原中存在38 kDa分子。
Zotero 插件