Akhtar Kafil, Sherwani Rana K, Sofi Lateef Ahmad, Sharma Ashish, Singh Prabhat
Department of Pathology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India.
Indian J Hematol Blood Transfus. 2009 Mar;25(1):27-9. doi: 10.1007/s12288-009-0006-6. Epub 2009 Apr 6.
Detection of Hook's effect, the false negative results in HBs Ag immunoassay screening. Two drops of donor's serum was added to the sample well of hepacard device using a dropper and was allowed to react for 20 minutes and results read thereafter. The hepacard device when read after 20 minutes showed only one distinct pink test line and no control line. Serial dilutions (1:10, 1: 20) of the donors sample in normal saline was done and the test was re-run. Sample in 1: 10 dilution showed both control and faint pink test line, which intensified to pink band in 1: 20 diluted sample. The goal in the immunoassay detection of HBsAg should be to minimize erroneous results; so as not to endanger patient health and the blood supply.
钩状效应的检测，即乙肝表面抗原免疫分析筛查中的假阴性结果。使用滴管将两滴供体血清加入肝牌检测装置的样本孔中，使其反应20分钟，然后读取结果。20分钟后读取时，肝牌检测装置仅显示一条明显的粉色检测线，无对照线。对供体样本在生理盐水中进行系列稀释（1:10、1:20），并重新进行检测。1:10稀释的样本显示出对照线和 faint粉色检测线，在1:20稀释的样本中检测线加深为粉色条带。乙肝表面抗原免疫分析检测的目标应该是尽量减少错误结果，以免危及患者健康和血液供应。 （注：“faint”原词拼写有误，可能是“faint”，这里按推测翻译）
