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免疫与分子检测方法用于诊断胃肠道病毒感染的比较。

Comparison of immunologic and molecular assays for the diagnosis of gastrointestinal viral infections.

机构信息

S.S. Virologia Molecolare, S.C. Virologia e Microbiologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

出版信息

Diagn Microbiol Infect Dis. 2013 Jan;75(1):110-1. doi: 10.1016/j.diagmicrobio.2012.09.016. Epub 2012 Oct 26.

Abstract

The performance of immunochromatographic and enzyme-linked immunosorbent assays (RIDA®QUICK and RIDASCREEN®, R-Biopharm) was compared with real-time reverse transcription-polymerase chain reaction techniques for the diagnosis of gastrointestinal viral infections. The sensitivity of the immunologic methods was comparable to polymerase chain reaction for the diagnosis of rotavirus and astrovirus, while it was reduced for detection of norovirus and adenovirus.

摘要

免疫层析和酶联免疫吸附测定(RIDA®QUICK 和 RIDASCREEN®,R-Biopharm)的性能与实时逆转录聚合酶链反应技术进行了比较,用于诊断胃肠道病毒感染。免疫方法的灵敏度与聚合酶链反应相当,可用于诊断轮状病毒和星状病毒,但用于检测诺如病毒和腺病毒时灵敏度降低。

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