用于二维晶体筛选的自动网格处理和图像采集。

Automated grid handling and image acquisition for two-dimensional crystal screening.

作者信息

Cheng Anchi

机构信息

Department of Cell Biology, The National Resource for Automated Molecular Microscopy, The Scripps Research Institute, La Jolla, CA, USA.

出版信息

Methods Mol Biol. 2013;955:297-305. doi: 10.1007/978-1-62703-176-9_16.

DOI:10.1007/978-1-62703-176-9_16

PMID:23132067

Abstract

Routine large-scale two-dimensional (2D) crystallization trials are good candidates for automation. For imaging the large number of grids prepared from 2D crystallization trials, a two-pass imaging protocol using Leginon and a grid handling robot is described. A manual target selection bridges the two imaging passes. The two passes can be combined into one if objects of interests at different stages of trials can be reliably found using automated methods.

摘要

常规大规模二维（2D）结晶试验很适合自动化操作。为了对二维结晶试验制备的大量网格进行成像，本文描述了一种使用Leginon和网格处理机器人的两遍成像方案。手动目标选择连接了这两遍成像。如果使用自动化方法能够可靠地找到试验不同阶段的感兴趣物体，那么这两遍成像可以合并为一遍。