在酿酒酵母细胞质中构建异丁醇生物合成的人工途径。
Construction of an artificial pathway for isobutanol biosynthesis in the cytosol of Saccharomyces cerevisiae.
作者信息
Matsuda Fumio, Kondo Takashi, Ida Kengo, Tezuka Hironori, Ishii Jun, Kondo Akihiko
机构信息
Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Kobe, Japan.
出版信息
Biosci Biotechnol Biochem. 2012;76(11):2139-41. doi: 10.1271/bbb.120420. Epub 2012 Nov 7.
To increase isobutanol production in Saccharomyces cerevisiae, the valine biosynthetic pathway was activated by overexpression of the relevant enzymes in the mitochondria and the cytosol. Native mitochondrial enzymes were overepxressed in the cytosol by deleting the mitochondrial transit peptides. The metabolically engineered S. cerevisiae possessing the cytosolic pathway showed increased isobutanol production (63 ± 4 mg/L).
为了提高酿酒酵母中异丁醇的产量，通过在线粒体和细胞质中过表达相关酶来激活缬氨酸生物合成途径。通过删除线粒体转运肽，将天然线粒体酶在细胞质中过表达。具有细胞质途径的代谢工程改造的酿酒酵母显示出异丁醇产量增加（63±4毫克/升）。
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