利用过量非同源片段提高构巢曲霉中的基因靶向效率。

Improvement of gene targeting in Aspergillus nidulans with excess non-homologous fragments.

作者信息

Matsuhira Kazuaki, Itoh Yasuo

机构信息

Graduate School of Science and Technology, Shinshu University, Matsumoto, Nagano 390-8621, Japan.

出版信息

Biosci Biotechnol Biochem. 2012;76(11):2146-9. doi: 10.1271/bbb.120456. Epub 2012 Nov 7.

DOI:10.1271/bbb.120456

PMID:23132572

Abstract

Efficiency of gene targeting was increased more than 10-fold when an excess molar ratio of non-homologous fragments was added in the transformation of Aspergillus nidulans. In the targeted transformants, integration of such fragments into the host chromosome was a rare event. Hence, our approach proved practical in terms of producing successful targeting events without disturbing the host chromosomes.

摘要

当在构巢曲霉转化过程中添加过量摩尔比的非同源片段时，基因靶向效率提高了10倍以上。在靶向转化体中，此类片段整合到宿主染色体中是罕见事件。因此，我们的方法在产生成功的靶向事件而不干扰宿主染色体方面证明是可行的。