[异基因造血干细胞移植后血液系统恶性肿瘤患者的额外核型异常分析]

[Additional karyotypic abnormalities analysis in patients with hematological malignancies post-allogeneic hematopoietic stem cell transplantation].

作者信息

Zhao Jia-wei, Zhai Wei-hua, Li Cheng-wen, Zhang Qin, Xu Fang-yun, Chen Heng-hua, Yue Jin-ya, Han Ming-zhe

机构信息

Institute of Hematology & Blood Diseases Hospital CAMS & PUMC, Tianjin, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2012 Aug;33(8):619-22.

PMID:23134853

Abstract

OBJECTIVE

To analyze the karyotype stability in hematological malignancies patients before and after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and its prognostic significance of monitoring.

METHODS

The karyotypes and clinical data of 21 patients with hematological malignancies at the initial diagnosis and at relapse after allo-HSCT were retrospectively reviewed. Chromosome analysis was performed by standard 24 h-cultured method and R banding.

RESULTS

Karyotypes at the initial diagnosis and at relapse after allo-HSCT were different in 11 patients (52.38%), including chromosome 1, 3, 6, 12, 17, 21. Numberical abnormalities and structural chromosomal abnormalities always occurs together. The median survival time of relapse of the patients with karyotype changes was significantly shorter than that of patients without a karyotype change (79 d vs 522 d, P = 0.027), and that of the patients with trisomy 6 was also significantly shorter than that of the patients without trisomy 6 (9 d vs 275 d, P = 0.005).

CONCLUSION

Karyotype changes after relapse are associated with the prognosis of allo-HSCT.

摘要

目的

分析异基因造血干细胞移植（allo-HSCT）前后血液系统恶性肿瘤患者的核型稳定性及其监测的预后意义。

方法

回顾性分析21例血液系统恶性肿瘤患者初次诊断时及allo-HSCT复发时的核型及临床资料。采用标准的24小时培养法和R显带技术进行染色体分析。

结果

11例患者（52.38%）初次诊断时与allo-HSCT复发时的核型不同，涉及染色体1、3、6、12、17、21。数目异常和结构染色体异常常同时出现。核型改变患者复发后的中位生存时间显著短于无核型改变患者（79天对522天，P = 0.027），6号染色体三体患者的中位生存时间也显著短于无6号染色体三体患者（9天对275天，P = 0.005）。