高盐度、高温和pH值对白斑综合征病毒衣壳结构的影响

Effects of high salinity, high temperature and pH on capsid structure of white spot syndrome virus.

作者信息

Chen Weiyu, Zhang Heng, Gu Li, Li Fang, Yang Feng

机构信息

College of Oceanography and Environmental Science of Xiamen University, PR China.

出版信息

Dis Aquat Organ. 2012 Nov 8;101(2):167-71. doi: 10.3354/dao02511.

DOI:10.3354/dao02511

PMID:23135144

Abstract

The structural stability of white spot syndrome virus (WSSV) capsids at high salinity, high temperature and various pH values was studied. To obtain the viral capsids, the nucleocapsids were treated with high salinity. The results showed that high salinity treatment can cause the dissociation of VP15 and most of VP95 from the nucleocapsid, but there were no noticeable alterations in morphology and ultrastructure of the nucleocapsid and capsid. The capsids retained morphological integrity at temperatures <45°C but became aberrant at >60°C. In addition, the capsids were relatively resistant to strong acid conditions and were tolerant to a broad pH range of 1 to 10. However, morphological change occurred at pH 10.5. The capsids broke up into small pieces at pH 11 and completely degraded in 0.1 and 1.0 M NaOH. These results indicated that the WSSV capsid is acid-stable and alkali-labile.

摘要

研究了白斑综合征病毒（WSSV）衣壳在高盐度、高温及不同pH值条件下的结构稳定性。为获得病毒衣壳，对核衣壳进行了高盐度处理。结果表明，高盐度处理可导致VP15和大部分VP95从核衣壳上解离，但核衣壳和衣壳的形态及超微结构无明显变化。衣壳在温度低于45°C时保持形态完整，但在高于60°C时变得异常。此外，衣壳对强酸条件相对耐受，在pH值1至10的较宽范围内都能耐受。然而，在pH值10.5时发生形态变化。衣壳在pH值11时破碎成小碎片，并在0.1和1.0 M NaOH中完全降解。这些结果表明，WSSV衣壳对酸稳定，对碱不稳定。