Schneider Steffen
BASF SE, Ludwigshafen, Germany.
Methods Mol Biol. 2013;947:215-21. doi: 10.1007/978-1-62703-131-8_17.
The assessment of developmental toxicology data is a critical aspect of hazard evaluation for pharmaceuticals and environmental chemicals. Skeletal examination is an essential part of prenatal developmental toxicity studies of chemicals as well as pesticides and comprises evaluation of both cartilaginous and ossified skeletal components. Various techniques are published in the literature to process and double-stain skeletons of common laboratory animals which are all based on staining of the cartilage with Alcian Blue and staining of ossified bones with Alizarin Red S along with maceration (clearing) of the surrounding soft tissue. The staining of the cartilage allows the examination to assess ossified structures and their underlying cartilage in a single step.
发育毒理学数据的评估是药物和环境化学品危害评估的关键方面。骨骼检查是化学品以及农药产前发育毒性研究的重要组成部分，包括对软骨和骨化骨骼成分的评估。文献中发表了各种处理和双重染色常见实验动物骨骼的技术，这些技术均基于用阿尔辛蓝对软骨进行染色，并用茜素红S对骨化骨骼进行染色，同时对周围软组织进行浸软（清除）处理。软骨染色可使检查在一个步骤中评估骨化结构及其下方的软骨。
