Lloyd R V, Jin L, Song J Y, Terry L C, Horvath E, Kovacs K
Department of Pathology, University of Michigan, Ann Arbor.
Lab Invest. 1990 Mar;62(3):347-54.
The effects of hypothyroidism induced by propylthiouracil (PTU) treatment on growth hormone (GH) and prolactin (PRL) messenger ribonucleic acid (mRNA) levels were analyzed in adult female rat adenohypophyses by in situ hybridization histochemistry and Northern hybridization analyses. Twenty-eight days of PTU treatment produced a significant decrease in GH mRNA levels and a smaller decrease in PRL mRNA determined by both in situ hybridization histochemistry and Northern hybridization analyses. A combined procedure of in situ hybridization histochemistry followed by immunochemistry on the same sections revealed mammosomatotropic cells expressing GH mRNA and PRL protein in the same pituitary cells from all treatment groups. Cells expressing GH mRNA and thyroid-stimulating hormone protein were not detected by this method. Immunochemical staining revealed a decrease in GH cells and an increase in thyroid-stimulating hormone cells in hypothyroid rats. Cells expressing both GH and thyroid-stimulating hormone protein were not detected by immunostaining. These results indicate that hypothyroidism produces significant decreases in GH mRNA and also decreases PRL mRNA and that mammosomatotropic cells can be detected in pituitaries from normal and hypothyroid rats.
通过原位杂交组织化学和Northern杂交分析，研究丙硫氧嘧啶（PTU）诱导的甲状腺功能减退对成年雌性大鼠腺垂体中生长激素（GH）和催乳素（PRL）信使核糖核酸（mRNA）水平的影响。PTU处理28天导致GH mRNA水平显著降低，通过原位杂交组织化学和Northern杂交分析确定PRL mRNA水平有较小程度的降低。在同一切片上进行原位杂交组织化学和免疫化学的联合程序显示，所有处理组的腺垂体细胞中均有表达GH mRNA和PRL蛋白的促乳腺生长细胞。通过该方法未检测到表达GH mRNA和促甲状腺激素蛋白的细胞。免疫化学染色显示甲状腺功能减退大鼠中GH细胞减少，促甲状腺激素细胞增加。免疫染色未检测到同时表达GH和促甲状腺激素蛋白的细胞。这些结果表明，甲状腺功能减退会导致GH mRNA显著降低，同时也会降低PRL mRNA，并且在正常和甲状腺功能减退大鼠的垂体中均可检测到促乳腺生长细胞。
