Wilks C R, Kogan V G
Aust Vet J. 1979 Aug;55(8):385-8.
An antiserum to a recent field isolate of infectious laryngotracheitis virus was conjugated with fluorescein isothiocyanate and used to detect viral antigen to infected chorioallantoic membranes and trachael epithelium by the direct fluorescent antibody technique. In experimentally infected birds, viral antigen was detected with the fluorescent antibody technique from 2 to 14 days post-inoculation but histological evidence of tracheitis was only observed from day three to day ten. The fluorescent antibody test detected 22 of 23 histologically confirmed cases of ILT and was more accurate than virus isolation when used in the diagnosis of respiratory disease from field outbreaks. It was concluded that the speed, accuracy and sensitivity of the fluorescent antibody technique make it a useful tool in the diagnosis of infectious laryngotracheitis.
将针对传染性喉气管炎病毒近期野外分离株的抗血清与异硫氰酸荧光素结合，通过直接荧光抗体技术用于检测感染的绒毛尿囊膜和气管上皮中的病毒抗原。在实验感染的禽类中，接种后2至14天用荧光抗体技术检测到病毒抗原，但气管炎症的组织学证据仅在第3天至第10天观察到。荧光抗体试验在23例经组织学确诊的传染性喉气管炎病例中检测出22例，在诊断野外暴发的呼吸道疾病时，其比病毒分离更准确。得出的结论是，荧光抗体技术的速度、准确性和敏感性使其成为诊断传染性喉气管炎的有用工具。
