粉尘螨诱导的早晚期哮喘反应中外周血 Th17 细胞和中性粒细胞。

Peripheral blood Th17 cells and neutrophils in Dermatophagoides pteronyssinus-induced early- and late-phase asthmatic response.

机构信息

Department of Pulmonology and Immunology, Medical Academy, Lithuanian University of Health Sciences, Eivenių 2, 50028 Kaunas, Lithuania.

出版信息

Medicina (Kaunas). 2012;48(9):442-51.

PMID:23168918

Abstract

BACKGROUND AND OBJECTIVE

Biphasic cellular immune reactions, which follow allergen inhalation, are a specific feature of inflammation in allergic asthma. The aim of this study was to determine the changes in the percentage of peripheral blood Th17 cells and neutrophil functions after Dermatophagoides pteronyssinus-induced early- and late-phase asthmatic response in patients with allergic asthma.

MATERIAL AND METHODS

A total of 19 patients with allergic asthma were examined. Eleven patients developed an isolated early-phase asthmatic response (EAR), whereas 8 developed both early- and late-phase (dual) asthmatic responses (DAR) after the bronchial challenge with Dermatophagoides pteronyssinus. The control group included 15 healthy subjects. Peripheral blood collection was performed 24 hours before as well as 7 and 24 hours after the bronchial challenge. The percentage of Th17 cells, and chemotaxis and apoptosis of neutrophils were analyzed by flow cytometry. The serum IL-8 and IL-17 levels were determined by ELISA.

RESULTS

After the bronchial challenge, the percentage of Th17 and IL-17 levels increased considerably 7 and 24 hours after the challenge in both groups of patients. Moreover, 24 hours after the challenge, the percentage of Th17 cells and IL-17 levels were significantly higher in the patients with the DAR than those with the EAR or healthy controls. Seven and 24 hours after the challenge, neutrophil chemotaxis was greater in the patients with the DAR as compared with those with the EAR and healthy controls as well. The apoptotic activity of neutrophils was lower 24 hours after the challenge in the patients with the DAR than those with the EAR.

CONCLUSIONS

Dermatophagoides pteronyssinus-induced early- and late-phase asthmatic response in patients with allergic asthma was found to be accompanied by an increased percentage of peripheral blood Th17 cells and elevated serum IL-17 levels as well as altered neutrophil functions.

摘要

背景和目的

双相细胞免疫反应是变应性哮喘炎症的一个特征，这种反应紧随过敏原吸入后发生。本研究的目的是确定变应性哮喘患者在变应原激发后早期和晚期相哮喘反应时外周血 Th17 细胞的百分比和中性粒细胞功能的变化。

材料和方法

共检查了 19 例变应性哮喘患者。11 例患者发生孤立的早期相哮喘反应（EAR），8 例患者在变应原激发后发生早期和晚期相（双重）哮喘反应（DAR）。对照组包括 15 例健康受试者。在支气管激发前 24 小时以及支气管激发后 7 小时和 24 小时采集外周血。通过流式细胞术分析 Th17 细胞的百分比、中性粒细胞的趋化和凋亡。通过 ELISA 测定血清 IL-8 和 IL-17 水平。

结果

支气管激发后，两组患者在激发后 7 小时和 24 小时 Th17 细胞百分比和 IL-17 水平均显著升高。而且，在激发后 24 小时，DAR 患者的 Th17 细胞百分比和 IL-17 水平显著高于 EAR 患者和健康对照组。激发后 7 小时和 24 小时，DAR 患者的中性粒细胞趋化作用较 EAR 患者和健康对照组更强。在 DAR 患者中，激发后 24 小时，中性粒细胞的凋亡活性低于 EAR 患者。