Si Tong, Liu Chunxiao, Xu Kai, Gui Yaoting
Department of Urinary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Nov;32(11):1568-71.
To investigate the association of miR-199a expression with the clinicopathologic characteristics and the survival of patients with renal cell carcinoma (RCC).
Real-time quantitative RT-PCR was used to detect the expression of miR-199a in the tumor tissues and paired adjacent normal tissues from 67 patients with RCC. The correlations of miR-199a expression with the clinicopathologic characteristics and survival of RCC patients were analyzed.
Positive miR-199a expression was detected in both the RCC and adjacent normal tissues, but the tumor tissues showed a significantly lower expression level by a mean of 13.7 folds (P=0.003). The expression of miR-199a was negatively correlated with tumor recurrence in RCC patients in T stage (P<0.05), but showed no significant correlations with the patients age, histological type of the tumor, lymph node metastasis, distal metastasis, or Fuhrman grade (P>0.05). The patients with lowered miR-199a expression in the tumor tissue had a significantly shorter mean survival time than those without miR-199a down-regulation (P=0.017 by Log-rank test).
A decreased expression of miR-199a is significantly correlated with a higher tumor stage, a greater likeliness of tumor recurrence, and a poorer prognosis in RCC patients. miR-199a can serve as a promising prognostic factor of renal cell carcinoma.
探讨miR-199a表达与肾细胞癌（RCC）患者临床病理特征及生存情况的相关性。
采用实时定量逆转录聚合酶链反应（RT-PCR）检测67例RCC患者肿瘤组织及配对的癌旁正常组织中miR-199a的表达。分析miR-199a表达与RCC患者临床病理特征及生存情况的相关性。
在RCC组织及癌旁正常组织中均检测到miR-199a阳性表达，但肿瘤组织中miR-199a表达水平显著降低，平均降低13.7倍（P=0.003）。miR-199a表达与RCC患者T分期的肿瘤复发呈负相关（P<0.05），但与患者年龄、肿瘤组织学类型、淋巴结转移、远处转移或Fuhrman分级无显著相关性（P>0.05）。肿瘤组织中miR-199a表达降低的患者平均生存时间显著短于miR-199a未下调的患者（Log-rank检验P=0.017）。
miR-199a表达降低与RCC患者肿瘤分期较高、肿瘤复发可能性较大及预后较差显著相关。miR-199a可作为肾细胞癌有前景的预后指标。
