[迷走神经切断术后大鼠迷走神经背运动核的逆行性改变]
[Retrograde changes in the dorsal motor nuclei of rat vagus nerve after vagotomy].
作者信息
Zhou Haiyang, Chang Yetian, Dai Ruping
机构信息
Department of Anesthesiology, Second Xiangya Hospital of Central South University, Changsha, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Nov;32(11):1576-9.
OBJECTIVE
To investigate the retrograde changes in the dorsal motor nuclei (DMV) of the vagus nerve after vagotomy in rats.
METHODS
Nissl staining and immunohistochemistry were used to observe the morphological and quantitative changes of the DMV and alterations of the expression of iNOS and NADPH after severing of the vagus nerve in adult male Wistar rats.
RESULTS
Compared with the control group, the rats with right vagotomy showed obvious morphological changes and a significantly decreased number of neurons in the right DMV (P<0.05). Numerous iNOS- and NADPH-immunopositive cells were detected in the right DMV 5 and 10 days after right vagotomy.
CONCLUSION
Vagotomy causes obvious retrograde changes in rat DMV shown by a significantly decreased number and obvious morphological changes of the neurons in the DMV.
目的
研究大鼠迷走神经切断术后迷走神经背核（DMV）的逆行性变化。
方法
采用尼氏染色和免疫组织化学方法，观察成年雄性Wistar大鼠迷走神经切断后DMV的形态和数量变化以及诱导型一氧化氮合酶（iNOS）和还原型辅酶Ⅱ（NADPH）表达的改变。
结果
与对照组相比，右侧迷走神经切断的大鼠右侧DMV出现明显的形态学变化，神经元数量显著减少（P<0.05）。右侧迷走神经切断后5天和10天，在右侧DMV中检测到大量iNOS和NADPH免疫阳性细胞。
结论
迷走神经切断术导致大鼠DMV出现明显的逆行性变化，表现为DMV中神经元数量显著减少和明显的形态学改变。
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