蛋白磷酸酶 2A Cα 通过调节成骨细胞中 RANKL 和 OPG 的表达参与破骨细胞的生成。
Protein phosphatase 2A Cα is involved in osteoclastogenesis by regulating RANKL and OPG expression in osteoblasts.
机构信息
Department of Histology and Oral Histology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, Tokushima 770-8504, Japan.
出版信息
FEBS Lett. 2013 Jan 4;587(1):48-53. doi: 10.1016/j.febslet.2012.10.041. Epub 2012 Nov 23.
We examined whether alteration of PP2A Cα expression in osteoblasts is involved in osteoclast differentiation. Reduction of PP2A Cα in MC3T3-E1 cells (shPP2A) decreased receptor activator of nuclear factor κB ligand (RANKL) expression and increased osteoprotegerin (OPG) expression. The conditioned medium from shPP2A cells failed to induce NFATc1 as well as the expression of osteoclast marker genes cathepsin K and osteoclast-associated receptor (OSCAR) in bone marrow macrophage cells. Treatment of bone marrow macrophage cells with the conditioned medium from shPP2A cells impaired osteoclastogenesis. These results suggest that alteration of PP2A Cα expression in osteoblasts modulates the expressions of RANKL and OPG, which are involved in osteoclastogenesis via the NFATc1 transcription factor.
我们研究了成骨细胞中蛋白磷酸酶 2A（PP2A）Cα 表达的改变是否参与破骨细胞分化。MC3T3-E1 细胞中 PP2A Cα 的减少（shPP2A）降低了核因子 κB 受体激活剂配体（RANKL）的表达，增加了护骨素（OPG）的表达。shPP2A 细胞的条件培养基不能诱导 NFATc1 以及破骨细胞标记基因组织蛋白酶 K 和破骨细胞相关受体（OSCAR）的表达。用 shPP2A 细胞的条件培养基处理骨髓巨噬细胞会损害破骨细胞的生成。这些结果表明，成骨细胞中 PP2A Cα 表达的改变通过 NFATc1 转录因子调节 RANKL 和 OPG 的表达，这涉及破骨细胞的生成。
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