猫和大鼠神经系统中二磷酸核苷消耗的组织化学特征

Histochemical characteristics of diphosphate nucleoside consumption in cat and rat nervous system.

作者信息

Martinez-Murillo R, Martinez-Rodriguez R, Toledano A, Barca M A

出版信息

Acta Histochem. 1979;65(2):138-45. doi: 10.1016/s0065-1281(79)80002-1.

DOI:10.1016/s0065-1281(79)80002-1

Abstract

Consumption of diphosphate nucleosides is investigated by histochemical methods in rat and cat nervous system. Results show NDP-ase is effectively in histological sections from animals previously perfused with glutaraldehyde or formaldehyde. Histochemical reaction is increased in presence of Ca++, Mg++, Mn++ or imidazole and inhibited by L-DOPA and Noradrenaline in incubation medium. A comparative study of TPP-ase and NDP-ase activities to dilucidate the identity of both enzymes is described.

摘要

采用组织化学方法研究大鼠和猫神经系统中二磷酸核苷的消耗情况。结果表明，在先前用戊二醛或甲醛灌注的动物组织切片中，NDP酶活性有效。在孵育介质中，Ca++、Mg++、Mn++或咪唑存在时组织化学反应增强，而L-多巴和去甲肾上腺素则抑制该反应。本文描述了一项关于TPP酶和NDP酶活性的比较研究，以阐明这两种酶的特性。

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