Boguta Magdalena
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02 106 Warsaw, Poland.
Biochim Biophys Acta. 2013 Mar-Apr;1829(3-4):376-84. doi: 10.1016/j.bbagrm.2012.11.004. Epub 2012 Nov 28.
tRNA synthesis by yeast RNA polymerase III (Pol III) is down-regulated under growth-limiting conditions. This control is mediated by Maf1, a global negative regulator of Pol III transcription. Conserved from yeast to man, Maf1 was originally discovered in Saccharomyces cerevisiae by a genetic approach. Details regarding the molecular basis of Pol III repression by Maf1 are now emerging from the recently reported structural and biochemical data on Pol III and Maf1. The phosphorylation status of Maf1 determines its nuclear localization and interaction with the Pol III complex and several Maf1 kinases have been identified to be involved in Pol III control. Moreover, Maf1 indirectly affects tRNA maturation and decay. Here I discuss the current understanding of the mechanisms that oversee the Maf1-mediated regulation of Pol III activity and the role of Maf1 in the control of tRNA biosynthesis in yeast. This article is part of a Special Issue entitled: Transcription by Odd Pols.
在生长受限条件下，酵母RNA聚合酶III（Pol III）介导的tRNA合成会下调。这种调控由Maf1介导，Maf1是Pol III转录的全局负调控因子。从酵母到人类，Maf1高度保守，最初是通过遗传方法在酿酒酵母中发现的。关于Maf1对Pol III抑制的分子基础的详细信息，现在正从最近报道的关于Pol III和Maf1的结构及生化数据中逐渐显现。Maf1的磷酸化状态决定其核定位以及与Pol III复合物的相互作用，并且已经鉴定出几种Maf1激酶参与Pol III的调控。此外，Maf1间接影响tRNA的成熟和降解。在此，我讨论了目前对监督Maf1介导的Pol III活性调控机制的理解，以及Maf1在酵母tRNA生物合成控制中的作用。本文是名为“奇特聚合酶的转录”特刊的一部分。
