UGC-DAE Consortium for Scientific Research, Trombay, Mumbai 400085, India.
Colloids Surf B Biointerfaces. 2013 Mar 1;103:267-74. doi: 10.1016/j.colsurfb.2012.10.034. Epub 2012 Oct 30.
The effects of electrostatic interaction between the hen egg white lysozyme (HEWL) and the functionalized iron oxide nanoparticles (IONPs) have been investigated using several techniques, e.g., CD, DSC, ζ-potential, UV-visible spectroscopy, DLS, TEM. Nanoparticles (IONPs) were functionalized with three hydrophilic ligands, viz., poly(ethylene glycol) (PEG), trisodium citrate (TSC) and sodium triphosphate (STP); where both TSC and STP contain Na(+) counter ions. It has been observed that the secondary structure of HEWL was not affected by PEG functionalized IONPs, but was partially and almost completely perturbed by TSC and STP functionalized IONPs, respectively. The perturbation of the secondary structure was irreversible. We have predicted an interaction model to explain the origin of perturbation of HEWL structure. We have also investigated the stability of nanoparticles dispersions after interaction with HEWL and used the DLVO theory to explain results.
使用多种技术，如圆二色性（CD）、差示扫描量热法（DSC）、ζ-电位、紫外-可见光谱法、动态光散射（DLS）、透射电子显微镜（TEM）等，研究了鸡卵清溶菌酶（HEWL）与功能化氧化铁纳米粒子（IONPs）之间静电相互作用的影响。纳米粒子（IONPs）通过三种亲水性配体进行功能化，即聚乙二醇（PEG）、柠檬酸三钠（TSC）和磷酸三钠（STP）；其中 TSC 和 STP 都含有 Na(+)抗衡离子。结果表明，PEG 功能化的 IONPs 不会影响 HEWL 的二级结构，但 TSC 和 STP 功能化的 IONPs 分别部分和几乎完全干扰 HEWL 的二级结构。这种二级结构的干扰是不可逆的。我们预测了一个相互作用模型来解释 HEWL 结构干扰的起源。我们还研究了纳米粒子分散体与 HEWL 相互作用后的稳定性，并使用 DLVO 理论来解释结果。
