单纯疱疹病毒温度敏感突变体的互补作用。
Complementation with ts mutants of herpes simplex virus.
作者信息
Messer L I, Timbury M C
出版信息
J Gen Virol. 1979 Nov;45(2):431-9. doi: 10.1099/0022-1317-45-2-431.
Tests to detect complementation between ts mutants of herpes simplex virus types 1 and 2 infectious centre and yield of virus assay were investigated. Progeny analysis of both intratypic and intertypic complementation showed a considerable proportion of recombinant or ts+ virus in the progeny; this was more marked in the infectious centre tests. Virus of intermediate temperature-sensitivity was produced in intratypic as well as intertypic complementation. Reduction in the input multiplicity of one of the two mutants in the test to extremely low levels did not prevent complementation, suggesting that non-infectious particles probably contribute to complementation. Demonstration of virus DNA synthesis in mixedly-infected cells at the non-permissive temperature was used to detect complementation between DNA-negative mutants.
对检测1型和2型单纯疱疹病毒温度敏感（ts）突变体之间互补作用的感染中心试验及病毒产量测定试验进行了研究。同型和异型互补的子代分析显示，子代中有相当比例的重组体或ts+病毒；这在感染中心试验中更为明显。在同型和异型互补中均产生了具有中等温度敏感性的病毒。将试验中两个突变体之一的输入复数降低到极低水平并不能阻止互补作用，这表明非感染性颗粒可能有助于互补作用。利用在非允许温度下混合感染细胞中病毒DNA合成的证明来检测DNA阴性突变体之间的互补作用。
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