Textile and Paper Engineering Department, Universitat Politècnica de Catalunya, Colom 11, E-08222 Terrassa, Spain.
Carbohydr Polym. 2013 Jan 30;92(1):276-82. doi: 10.1016/j.carbpol.2012.08.083. Epub 2012 Aug 30.
Various combinations of laccases, xylanase and cellulase were used to biobleach cellulose fibres from eucalyptus. The Trametes villosa and Myceliophthora thermophila laccases were used in combination with violuric acid (VA(TvL) system) and methyl syringate (MeS(MtL) system), respectively, as mediator. A dissimilar mode of action of the two systems was found: the VA(TvL) treatment released both hexenuronic acids and lignin, whereas the MeS(MtL) released lignin alone. Pulp properties were further improved by applying the mediator before the enzyme during treatment. Pulp properties comparable to those provided by industrial TCF sequences were obtained by inserting a xylanase pretreatment before VA(TvL), but no significant effect was observed after the cellulase pretreatment. As an added value, the resulting enzymatically bleached fibres possess a reduced hexenuronic acid content. The chemical oxygen demand of the effluents from each stage was also assessed.
各种漆酶、木聚糖酶和纤维素酶的组合被用于从桉树中生物漂白纤维素纤维。使用木蹄层孔菌漆酶和嗜热毁丝霉漆酶分别与均苯三酚（VA（TvL）系统）和甲基丁香酚（MeS（MtL）系统）结合作为介体。发现这两个系统的作用模式不同：VA（TvL）处理释放了戊糖醛酸和木质素，而 MeS（MtL）仅释放木质素。在处理过程中，在酶之前应用介体可以进一步改善纸浆性能。通过在 VA（TvL）之前进行木聚糖酶预处理，可以获得与工业 TCF 序列相当的纸浆性能，但在纤维素酶预处理后没有观察到显著效果。作为附加值，所得酶漂白纤维的戊糖醛酸含量降低。还评估了每个阶段流出物的化学需氧量。
