Center for Environmental Research and Technology, Bourns College of Engineering, University of California, Riverside, 1084 Columbia Avenue, Riverside, CA 92507, United States.
Carbohydr Polym. 2013 Jan 30;92(1):334-44. doi: 10.1016/j.carbpol.2012.09.070. Epub 2012 Oct 2.
Dilute oxalic acid pretreatment was applied to maple wood to improve compatibility with downstream operations, and its performance in pretreatment and subsequent enzymatic hydrolysis was compared to results for hydrothermal and dilute hydrochloric and sulfuric acid pretreatments. The highest total xylose yield of ∼84% of the theoretical maximum was for both 0.5% oxalic and sulfuric acid pretreatment at 160 °C, compared to ∼81% yield for hydrothermal pretreatment at 200 °C and for 0.5% hydrochloric acid pretreatment at 140 °C. The xylooligomer fraction from dilute oxalic acid pretreatment was only 6.3% of the total xylose in solution, similar to results with dilute hydrochloric and sulfuric acids but much lower than the ∼70% value for hydrothermal pretreatment. Combining any of the four pretreatments with enzymatic hydrolysis with 60 FPU cellulase/g of glucan plus xylan in the pretreated maple wood resulted in virtually the same total glucose plus xylose yields of ∼85% of the maximum possible.
草酸稀释预处理应用于枫木，以提高与下游操作的兼容性，并将其预处理和随后的酶解性能与水热预处理、稀盐酸和稀硫酸预处理的结果进行比较。在 160°C 下，0.5%的草酸和硫酸预处理的总木糖得率最高，达到理论最大值的 84%，而在 200°C 下的水热预处理和在 140°C 下的 0.5%盐酸预处理的总木糖得率约为 81%。与稀盐酸和稀硫酸的结果相似，但远低于水热预处理的约 70%，来自稀草酸预处理的木寡糖部分仅占溶液中总木糖的 6.3%。将这四种预处理中的任意一种与酶解相结合，在预处理的枫木中用 60 FPU 纤维素酶/克的葡聚糖加木聚糖进行酶解，最终得到的总葡萄糖加木糖得率几乎相同，均约为最大可能值的 85%。
