Concejo Nacional de Ciencia y Tecnología, Argentina.
Carbohydr Polym. 2013 Jan 30;92(1):817-23. doi: 10.1016/j.carbpol.2012.09.094. Epub 2012 Oct 6.
Many studies have focused on seed decontamination but no one has been capable of eliminating all pathogenic bacteria. Two objectives were followed. First, to assess the in vitro antimicrobial activity of chitosan against: (a) Escherichia coli O157:H7, (b) native microflora of lettuce and (c) native microflora of lettuce seeds. Second, to evaluate the efficiency of chitosan on reducing microflora on lettuce seeds. The overall goal was to find a combination of contact time and chitosan concentration that reduces the microflora of lettuce seeds, without affecting germination. After treatment lettuce seeds presented no detectable microbial counts (<10(2)CFU/50 seeds) for all populations. Moreover, chitosan eliminated E. coli. Regardless of the reduction in the microbial load, a 90% reduction on germination makes imbibition with chitosan, uneconomical. Subsequent treatments identified the optimal treatment as 10 min contact with a 10 g/L chitosan solution, which maintained the highest germination percentage.
许多研究都集中在种子消毒上，但没有人能够彻底消除所有致病菌。本研究有两个目的。首先，评估壳聚糖对以下物质的体外抗菌活性：(a)大肠杆菌 O157:H7，(b)生菜原生菌群，(c)生菜种子原生菌群。其次，评估壳聚糖降低生菜种子菌群的效率。总的目标是找到一种既能减少生菜种子菌群，又不影响发芽的接触时间和壳聚糖浓度组合。经过处理后，所有种群的生菜种子的微生物计数均低于检测限(<10(2)CFU/50 颗种子)。此外，壳聚糖还能消灭大肠杆菌。尽管微生物负荷减少了，但发芽率降低 90%，使得壳聚糖的浸种处理不经济。进一步的处理确定了最佳处理方法是用 10 g/L 的壳聚糖溶液处理 10 分钟，该方法能保持最高的发芽率。
