Browitt Nanoparticle Laboratory, Department of Applied Mathematics, Research School of Physical Sciences and Engineering, Australian National University, Canberra ACT 0200, Australia.
Biomaterials. 2013 Feb;34(6):1732-8. doi: 10.1016/j.biomaterials.2012.11.022. Epub 2012 Dec 4.
Diagnostic imaging of the blood perfusion of the lungs is currently performed using particles of macro-aggregated albumin, which are mechanically arrested at limiting diameters of the capillary bed. While the proportion of blood flow obstructed is typically very low and temporary, it would seem more desirable to image lung perfusion in patients using a non-obstructive method, and using materials that avoid biological hazards. We have characterised the in vitro and in vivo properties of a colloid of a cationised radiolabelled nanocomposite. The nanoparticles comprise a Technetium-99m core encapsulated in graphitic carbon, and coated with low molecular weight poly-lysine to provide a strong charge-based affinity for the endothelial glycocalyx of the lung. Following intravenous injection in rabbits and cats, the nanoparticles rapidly distribute and localise in the lungs, thus enabling gamma camera imaging of lung perfusion. Repeat administration of this colloid in both species over several weeks indicates favourable biocompatibility.
目前，肺部血液灌注的诊断成像采用的是大聚合白蛋白颗粒，这些颗粒在毛细血管床的极限直径处被机械截留。虽然被阻塞的血流比例通常非常低且是暂时的，但使用非阻塞性方法并使用避免生物危害的材料来对患者的肺灌注进行成像似乎更为理想。我们已经对一种带正电荷的放射性标记纳米复合材料胶体的体外和体内特性进行了描述。这些纳米颗粒由包裹在石墨碳中的锝-99m 核心组成，并涂有低分子量聚赖氨酸，以提供与肺内皮糖萼的强烈基于电荷的亲和力。在兔子和猫中静脉注射后，纳米颗粒迅速分布并在肺部定位，从而能够进行肺灌注的伽马相机成像。在数周内，在这两种物种中重复给予这种胶体表明其具有良好的生物相容性。
