Animal Health Biotechnology, Temasek Life Sciences Laboratory, Singapore.
J Virol Methods. 2013 Mar;188(1-2):76-82. doi: 10.1016/j.jviromet.2012.11.038. Epub 2012 Dec 7.
Hand, foot and mouth disease (HFMD) is a viral infectious disease caused by human Enterovirus A, particularly Enterovirus 71 (EV71) and Coxsackievirus 16 (CA16) serotypes, with EV71 infection associated with severe neurological complications and mortality. Lots of attention has been placed on elucidating viral epitopes, which is useful for EV71 viral research. In this study, a murine monoclonal antibody (mAb 4) specific for EV71 was generated and mapped to target the N-terminal region of VP1 capsid protein, spanning amino acid residues 12-19 (IGDSVSRA). mAb 4 can cross-react with all the 11 representative EV71 subgenotypes (A, B1-5, C1-5), but not with the representative strain of CA16 as demonstrated by immunofluorescence assay (IFA). BLAST analyses of this epitope against all Enterovirus entries in Genbank also demonstrated that this epitope is unique in EV71, but not other Enterovirus such as CA16 It may be useful for structural study of VP1 morphogenesis during infection and also applications for identification of EV71 infection.
手足口病（HFMD）是一种由肠道病毒 A 引起的病毒性传染病，特别是肠道病毒 71 型（EV71）和柯萨奇病毒 16 型（CA16）血清型，EV71 感染与严重的神经并发症和死亡率有关。人们已经对阐明病毒表位给予了很多关注，这对于 EV71 病毒的研究很有用。在这项研究中，产生了一种针对 EV71 的鼠单克隆抗体（mAb4），并将其定位到衣壳蛋白 VP1 的 N 端区域，跨越氨基酸残基 12-19（IGDSVSRA）。mAb4 可以与所有 11 种代表性 EV71 亚属（A、B1-5、C1-5）发生交叉反应，但不能与免疫荧光测定（IFA）所示的 CA16 代表株发生反应。对 Genbank 中所有肠道病毒条目进行的该表位的 BLAST 分析也表明，该表位在 EV71 中是独特的，但在其他肠道病毒如 CA16 中则不是。它可能有助于研究感染过程中 VP1 形态发生的结构，也有助于鉴定 EV71 感染。
