简化陷阱。
Simplification traps.
作者信息
Prinz Heino
机构信息
Max-Planck-Institut für molekulare Physiologie, Otto-Hahn-Str. 11, 44227 Dortmund, Germany.
出版信息
J Chem Biol. 2012 Jan;5(1):1-4. doi: 10.1007/s12154-011-0069-3. Epub 2011 Dec 7.
UNLABELLED
When experiments are analyzed with simple functions, one gets simple results. A trap springs when experiments show deviations from the expected simplicity. When kinetic experiments do not follow exponential curves, they simply are not of the first or pseudofirst order. They can and have to be calculated on the base of plausible reaction schemes. When dose-response curves are analyzed with logistic functions ("4-parameter fit") and give Hill coefficients different from one, this is an experimental result stating that more than one molecule is involved in eliciting the response. If one ignores that result, one usually finds forgiving referees, but one will loose real money when one tries to develop such an unspecific compound into a drug.
ELECTRONIC SUPPLEMENTARY MATERIAL
The online version of this article (doi:10.1007/s12154-011-0069-3) contains supplementary material, which is available to authorized users.
未标注
当用简单函数分析实验时，会得到简单的结果。当实验显示出与预期的简单性存在偏差时，问题就出现了。当动力学实验不遵循指数曲线时，它们根本就不是一级或准一级反应。它们可以而且必须基于合理的反应机制来计算。当用逻辑函数（“四参数拟合”）分析剂量反应曲线并得出不同于1的希尔系数时，这是一个实验结果，表明引发反应涉及不止一个分子。如果忽视这个结果，通常会遇到宽容的审稿人，但当试图将这种非特异性化合物开发成药物时，就会损失实实在在的金钱。
电子补充材料
本文的在线版本（doi:10.1007/s12154-011-0069-3）包含补充材料，授权用户可以获取。
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