RNA 解旋酶在剪接中的作用。
RNA helicases in splicing.
机构信息
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK.
出版信息
RNA Biol. 2013 Jan;10(1):83-95. doi: 10.4161/rna.22547. Epub 2012 Dec 10.
Abstract
In eukaryotic cells, introns are spliced from pre-mRNAs by the spliceosome. Both the composition and the structure of the spliceosome are highly dynamic, and eight DExD/H RNA helicases play essential roles in controlling conformational rearrangements. There is evidence that the various helicases are functionally and physically connected with each other and with many other factors in the spliceosome. Understanding the dynamics of those interactions is essential to comprehend the mechanism and regulation of normal as well as of pathological splicing. This review focuses on recent advances in the characterization of the splicing helicases and their interactions, and highlights the deep integration of splicing helicases in global mRNP biogenesis pathways.
摘要
在真核细胞中，内含子通过剪接体从 pre-mRNAs 中被剪接。剪接体的组成和结构都是高度动态的，八个 DExD/H RNA 解旋酶在控制构象重排方面发挥着重要作用。有证据表明，各种解旋酶在功能上和物理上与剪接体中的许多其他因子相互连接。了解这些相互作用的动态对于理解正常和病理剪接的机制和调节至关重要。本综述重点介绍了对剪接解旋酶及其相互作用的特征描述的最新进展，并强调了剪接解旋酶在全局 mRNP 生物发生途径中的深度整合。
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