RNA 解旋酶在剪接中的作用。
RNA helicases in splicing.
机构信息
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK.
出版信息
RNA Biol. 2013 Jan;10(1):83-95. doi: 10.4161/rna.22547. Epub 2012 Dec 10.
Abstract
In eukaryotic cells, introns are spliced from pre-mRNAs by the spliceosome. Both the composition and the structure of the spliceosome are highly dynamic, and eight DExD/H RNA helicases play essential roles in controlling conformational rearrangements. There is evidence that the various helicases are functionally and physically connected with each other and with many other factors in the spliceosome. Understanding the dynamics of those interactions is essential to comprehend the mechanism and regulation of normal as well as of pathological splicing. This review focuses on recent advances in the characterization of the splicing helicases and their interactions, and highlights the deep integration of splicing helicases in global mRNP biogenesis pathways.
摘要
在真核细胞中，内含子通过剪接体从 pre-mRNAs 中被剪接。剪接体的组成和结构都是高度动态的，八个 DExD/H RNA 解旋酶在控制构象重排方面发挥着重要作用。有证据表明，各种解旋酶在功能上和物理上与剪接体中的许多其他因子相互连接。了解这些相互作用的动态对于理解正常和病理剪接的机制和调节至关重要。本综述重点介绍了对剪接解旋酶及其相互作用的特征描述的最新进展，并强调了剪接解旋酶在全局 mRNP 生物发生途径中的深度整合。
相似文献
1
RNA helicases in splicing.
RNA Biol. 2013 Jan;10(1):83-95. doi: 10.4161/rna.22547. Epub 2012 Dec 10.
2
Structure, function and regulation of spliceosomal RNA helicases.
Curr Opin Cell Biol. 2012 Jun;24(3):431-8. doi: 10.1016/j.ceb.2012.03.004. Epub 2012 Mar 29.
3
DEAH-Box RNA Helicases in Pre-mRNA Splicing.
Trends Biochem Sci. 2021 Mar;46(3):225-238. doi: 10.1016/j.tibs.2020.10.006. Epub 2020 Nov 30.
4
Functional roles of DExD/H-box RNA helicases in Pre-mRNA splicing.
J Biomed Sci. 2015 Jul 16;22(1):54. doi: 10.1186/s12929-015-0161-z.
5
The DEAH box ATPases Prp16 and Prp43 cooperate to proofread 5' splice site cleavage during pre-mRNA splicing.
Mol Cell. 2010 Aug 13;39(3):385-95. doi: 10.1016/j.molcel.2010.07.014.
6
Helicases involved in splicing from malaria parasite Plasmodium falciparum.
Parasitol Int. 2011 Dec;60(4):335-40. doi: 10.1016/j.parint.2011.09.007. Epub 2011 Oct 1.
7
DEAH-box ATPase Prp16 has dual roles in remodeling of the spliceosome in catalytic steps.
RNA. 2011 Jan;17(1):145-54. doi: 10.1261/rna.2459611. Epub 2010 Nov 22.
8
Splicing fidelity: DEAD/H-box ATPases as molecular clocks.
RNA Biol. 2013 Jul;10(7):1073-9. doi: 10.4161/rna.25245. Epub 2013 Jun 3.
9
A central role of Cwc25 in spliceosome dynamics during the catalytic phase of pre-mRNA splicing.
RNA. 2017 Apr;23(4):546-556. doi: 10.1261/rna.059204.116. Epub 2017 Jan 5.
10
Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2.
Genes Dev. 2005 Dec 15;19(24):2991-3003. doi: 10.1101/gad.1377405.
引用本文的文献
1
New mechanistic insights into Prp22-mediated exon ligation and mRNA release.
Nucleic Acids Res. 2025 Aug 27;53(16). doi: 10.1093/nar/gkaf823.
2
Brr2p-mediated unwinding of U4/U6 is promoted by a mutually exclusive intra-molecular stem loop in U4 and involves destabilization of the 5' stem-loop of U4.
bioRxiv. 2025 Aug 2:2025.08.01.667943. doi: 10.1101/2025.08.01.667943.
3
Nascent RNA Folding and RNP Assembly Revealed by Single-molecule Microscopy.
J Mol Biol. 2025 Aug 7:169365. doi: 10.1016/j.jmb.2025.169365.
4
Determination of trunk neural crest cell fate and susceptibility to splicing perturbation by the DLC1-SF3B1-PHF5A splicing complex.
Nat Commun. 2025 Jul 21;16(1):6718. doi: 10.1038/s41467-025-62003-6.
5
Role of DEAD/DEAH-box helicases in immunity, infection and cancers.
Cell Commun Signal. 2025 Jun 19;23(1):292. doi: 10.1186/s12964-025-02225-9.
6
Loss of drives impairments in tendon structure and function.
Matrix Biol Plus. 2025 Feb 15;25:100168. doi: 10.1016/j.mbplus.2025.100168. eCollection 2025 Feb.
7
Structural insights into spliceosome fidelity: DHX35-GPATCH1- mediated rejection of aberrant splicing substrates.
Cell Res. 2025 Apr;35(4):296-308. doi: 10.1038/s41422-025-01084-w. Epub 2025 Feb 28.
8
Knockout of Causes Inner Ear Developmental Defects in Zebrafish.
Biomedicines. 2024 Dec 26;13(1):20. doi: 10.3390/biomedicines13010020.
9
Structures of aberrant spliceosome intermediates on their way to disassembly.
Nat Struct Mol Biol. 2025 May;32(5):914-925. doi: 10.1038/s41594-024-01480-7. Epub 2025 Jan 20.
10
UBL5 and Its Role in Viral Infections.
Viruses. 2024 Dec 16;16(12):1922. doi: 10.3390/v16121922.
本文引用的文献
1
The Prp8 RNase H-like domain inhibits Brr2-mediated U4/U6 snRNA unwinding by blocking Brr2 loading onto the U4 snRNA.
Genes Dev. 2012 Nov 1;26(21):2422-34. doi: 10.1101/gad.200949.112.
2
Brr2p-mediated conformational rearrangements in the spliceosome during activation and substrate repositioning.
Genes Dev. 2012 Nov 1;26(21):2408-21. doi: 10.1101/gad.199307.112.
3
Structural basis for functional cooperation between tandem helicase cassettes in Brr2-mediated remodeling of the spliceosome.
Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17418-23. doi: 10.1073/pnas.1208098109. Epub 2012 Oct 8.
4
Structural bioinformatics of the human spliceosomal proteome.
Nucleic Acids Res. 2012 Aug;40(15):7046-65. doi: 10.1093/nar/gks347. Epub 2012 May 9.
5
Staying on message: ensuring fidelity in pre-mRNA splicing.
Trends Biochem Sci. 2012 Jul;37(7):263-73. doi: 10.1016/j.tibs.2012.04.001. Epub 2012 May 5.
6
Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS.
RNA. 2012 Jun;18(6):1244-56. doi: 10.1261/rna.033316.112. Epub 2012 Apr 25.
7
The spliceosome: a flexible, reversible macromolecular machine.
Trends Biochem Sci. 2012 May;37(5):179-88. doi: 10.1016/j.tibs.2012.02.009. Epub 2012 Apr 3.
8
A quantitative, high-throughput reverse genetic screen reveals novel connections between Pre-mRNA splicing and 5' and 3' end transcript determinants.
PLoS Genet. 2012;8(3):e1002530. doi: 10.1371/journal.pgen.1002530. Epub 2012 Mar 29.
9
Structure, function and regulation of spliceosomal RNA helicases.
Curr Opin Cell Biol. 2012 Jun;24(3):431-8. doi: 10.1016/j.ceb.2012.03.004. Epub 2012 Mar 29.
10
Dynamic protein-protein interaction wiring of the human spliceosome.
Mol Cell. 2012 Feb 24;45(4):567-80. doi: 10.1016/j.molcel.2011.12.034.
Zotero 插件