Zhou Zhen-yu, Tu Kang-sheng, Zhang Jing, Zheng Xin, Gao Jie, Yao Ying-min, Liu Qing-guang
Department of Hepatobiliary Surgery, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Dec;28(12):1303-6.
To observe the expression of Fbxw7 and explore its correlation with cell proliferation of hepatocellular carcinoma (HCC).
The expression of Fbxw7 in paired tumor and tumor-adjacent normal tissues from 40 patients with HCC were assessed using RT-PCR and immunohistochemistry. The expression level of Fbxw7 mRNA in normal liver cell line (LO2) and HCC cell lines (Hep3B and SMMC-7721) were detected by real-time RT-PCR. Colony formation assay and tumor xenograft assay were performed in different cell lines with different Fbxw7 expression respectively to investigate the ability of cell proliferation in vitro and in vivo.
The expression of Fbxw7 at both mRNA and protein levels in HCC tissues were significantly down-regulated compared to tumor-adjacent normal tissues (P<0.05). Down-regulation of Fbxw7 was found apparently associated with high Edmonson-Steiner classification and advanced TNM stage (P<0.05). The expression of Fbxw7 mRNA in LO2 was significantly higher than that in Hep3B or SMMC-7721 (P<0.05). Cell lines with less Fbxw7 expression had not only more colonies in colony formation assay (P<0.05), but also bigger xenografts in vitro (P<0.05).
The low expression of Fbxw7 correlates with the poor clinicopathological characteristics and cell proliferation of HCC.
观察Fbxw7的表达情况，并探讨其与肝细胞癌（HCC）细胞增殖的相关性。
采用RT-PCR和免疫组织化学方法检测40例HCC患者肿瘤组织及癌旁正常组织中Fbxw7的表达。通过实时RT-PCR检测正常肝细胞系（LO2）和HCC细胞系（Hep3B和SMMC-7721）中Fbxw7 mRNA的表达水平。分别在不同Fbxw7表达的细胞系中进行集落形成实验和肿瘤异种移植实验，以研究细胞在体外和体内的增殖能力。
与癌旁正常组织相比，HCC组织中Fbxw7的mRNA和蛋白水平均显著下调（P<0.05）。Fbxw7的下调明显与高Edmonson-Steiner分级和晚期TNM分期相关（P<0.05）。LO2中Fbxw7 mRNA的表达明显高于Hep3B或SMMC-7721（P<0.05）。Fbxw7表达较少的细胞系在集落形成实验中不仅有更多的集落（P<0.05），而且在体外异种移植中肿瘤更大（P<0.05）。
Fbxw7的低表达与HCC的不良临床病理特征和细胞增殖相关。
Zotero 插件