Bayati A
Department of Physiology and Medical Biophysics, University of Uppsala, Sweden.
Acta Physiol Scand. 1990 Mar;138(3):349-57. doi: 10.1111/j.1748-1716.1990.tb08857.x.
The fate of the trapped deformed erythrocytes seen in the early recirculation phase after ischaemia and the generation of long Tamm-Horsfall (TH) cylinders in the renal medulla during the first week after recirculation was studied in rats. In an in-vitro study the effects of different concentrations of TH protein on the permeability to Na+ of a semipermeable membrane were also investigated. The trapping of erythrocytes was found to be a reflow phenomenon, as there was no increase in the capillary area of the medulla in kidneys subjected to ischaemia but with no recirculation. This area increased to a maximum of 34.6 +/- 2.07% 20 min after recirculation and decreased to a normal value of 3.3 +/- 0.74% 1 day after the primary ischaemia. The area occupied by cylinders increased to a maximum of 19.2 +/- 1.4% 2 days after the primary damage and was as large as 16.7 +/- 1.47% after 1 week. It was also shown that the diffusion half-time of Na+ ions across a semipermeable membrane increased from 11.4 +/- 0.45 min to a maximum of 32.2 +/- 2.19 min with a protein concentration of 1 mg ml-1. It was concluded that the trapping of erythrocytes alone could not explain the decrease in renal function 1 week after the primary damage, but that the blockade of the tubules by the long homogeneous TH cylinders could be responsible for this decrease.
研究了大鼠缺血后早期再循环阶段所见被困变形红细胞的命运以及再循环后第一周肾髓质中长Tamm-Horsfall（TH）管型的生成情况。在一项体外研究中，还研究了不同浓度的TH蛋白对半透膜Na⁺通透性的影响。发现红细胞的滞留是一种回流现象，因为在遭受缺血但未进行再循环的肾脏中，髓质的毛细血管面积没有增加。该面积在再循环后20分钟增加到最大值34.6±2.07%，在初次缺血后1天降至正常值3.3±0.74%。管型所占面积在初次损伤后2天增加到最大值19.2±1.4%，1周后为16.7±1.47%。还表明，当蛋白质浓度为1mg/ml时，Na⁺离子跨半透膜的扩散半衰期从11.4±0.45分钟增加到最大值32.2±2.19分钟。得出的结论是，仅红细胞的滞留不能解释初次损伤后1周肾功能的下降，而长均质TH管型对肾小管的阻塞可能是导致这种下降的原因。
