Teodoro L O, Melo-Junior A A, Spercoski K M, Morais R N, Souza F F
Scientific Conservation Breeding Center, Companhia Brasileira de Metalurgia e Mineração - CBMM, Araxá, MG, Brazil.
Reprod Domest Anim. 2012 Dec;47 Suppl 6:250-5. doi: 10.1111/rda.12071.
The aim of the study was to evaluate the seasonality of andrological characteristics and hormonal profile of captive maned wolves (Chrysocyon brachyurus, Illiger 1811). Three adult males were evaluated from the Companhia Brasileira de Metalurgia e Mineração Scientific Breeding Center in Araxá, MG, Brazil, over 13 months. Semen was collected 2-3 times weekly and analysed. Scrotal circumference, biometrics and testicular volume were measured. Stool samples were collected 2-3 times weekly to analyse corticosteroid and testosterone metabolite concentrations. A success rate of 100% was achieved in the collection attempts during the breeding season (BS) and 77.8% during the non-breeding season (NBS). The interval to achieve penile erection was 1-5 min in the BS and 6-10 in the NBS (p < 0.001). Of the ejaculates collected, 80.0% contained sperm during BS, while 28.6% did during the NBS. The ejaculate had only one fraction, was odourless, predominantly translucent (72.4%), with a watery appearance, pH 6.7 and osmolarity of 352.8 mOsmol. Seasonal influences were seen in ejaculate volume (1.3 ml vs 0.4 ml), number of spermatozoa per ejaculate (73.9 × 10(6) vs 6.1 × 10(6) ) and percentage of live sperm (82.0% vs 66.1%) between the BS and NBS (p < 0.05), respectively. A high percentage of major sperm defects were observed in both seasons (50.1% in BS; 65.7% in NBS). Testicular volume was larger (p < 0.05; right testicles 13.1 cm(3) in BS vs 4.0 cm(3) in NBS, while left testicles 12.9 cm(3) in BS vs 5.3 cm(3) in NBS) and testicular consistency increased in the BS. No difference was seen in the basal faecal metabolite concentrations of testosterone; however, the corticosteroid concentrations were higher in the BS. Based on these results, it is possible to conclude that the collection of semen is feasible in captive maned wolves without compromising libido, seminal characteristics and reproductive behaviour and that sperm production is influenced by seasonality; however, it appears that there is no seasonal influence on basal testosterone concentrations.
本研究的目的是评估圈养鬃狼（Chrysocyon brachyurus，伊利格，1811年）的雄性生殖特征和激素水平的季节性变化。在巴西米纳斯吉拉斯州阿拉萨的巴西金属与矿业公司科学繁育中心，对三只成年雄性鬃狼进行了为期13个月的评估。每周采集2 - 3次精液并进行分析。测量阴囊周长、生物特征和睾丸体积。每周采集2 - 3次粪便样本，分析皮质类固醇和睾酮代谢物浓度。繁殖季节（BS）精液采集成功率为100%，非繁殖季节（NBS）为77.8%。繁殖季节阴茎勃起的间隔时间为1 - 5分钟，非繁殖季节为6 - 10分钟（p < 0.001）。在采集的精液中，繁殖季节有80.0%含有精子，而非繁殖季节为28.6%。射精只有一部分，无气味，主要为半透明（72.4%），外观似水状，pH值为6.7，渗透压为352.8毫摩尔渗透压浓度。繁殖季节和非繁殖季节的射精量（1.3毫升对0.4毫升）、每次射精的精子数量（73.9×10⁶对6.1×10⁶）和活精子百分比（82.0%对66.1%）存在季节性影响（p < 0.05）。两个季节均观察到较高比例的主要精子缺陷（繁殖季节为50.1%；非繁殖季节为65.7%）。睾丸体积更大（p < 0.05；繁殖季节右侧睾丸为13.1立方厘米，非繁殖季节为4.0立方厘米，左侧睾丸繁殖季节为12.9立方厘米，非繁殖季节为5.3立方厘米），且繁殖季节睾丸质地增加。睾酮的基础粪便代谢物浓度没有差异；然而，繁殖季节的皮质类固醇浓度更高。基于这些结果，可以得出结论，圈养鬃狼采集精液是可行的，不会损害性欲、精液特征和生殖行为，精子产生受季节性影响；然而，基础睾酮浓度似乎不受季节性影响。
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