Departament of Imunology, Aggeu Magalhães Research Center - Fiocruz, Recife, Pernambuco, Brazil.
J Appl Microbiol. 2013 Apr;114(4):1103-8. doi: 10.1111/jam.12119. Epub 2013 Jan 11.
Evaluate the IS6110-Taqman system performance in sputum samples from patients with pulmonary tuberculosis from health services in north-eastern Brazil as a diagnostic laboratory tool for pulmonary tuberculosis.
165 sputum samples from respiratory symptomatic patients were evaluated in the IS6110-TaqMan assay: 66 patients with pulmonary tuberculosis and 99 without TB. When the IS6110-TaqMan assay was evaluated using culture and/or clinical response to the specific treatment as the gold standard, IS6110-TaqMan assay obtained a sensitivity of 87.9% and specificity of 98%. The performance of IS6110-TaqMan assay was also evaluated with the sputum smear microscopy, resulting in a sensitivity of 79.7% and specificity 94.8%.
The IS6110-TaqMan was rapid, sensitive and specific for the diagnosis of pulmonary TB.
IS6110-TaqMan assay is a promising auxiliary tool for the diagnosis of pulmonary TB when used in conjunction with routine laboratory tests, clinical and epidemiological criteria of the patient, thus increasing the sensitivity and specificity of diagnosis.
评估 IS6110-Taqman 系统在巴西东北部卫生服务机构肺结核患者的痰样本中的性能，作为肺结核诊断实验室工具。
在 IS6110-TaqMan 检测中评估了 165 份呼吸道症状患者的痰样本：66 例肺结核患者和 99 例无结核病患者。当以培养和/或对特定治疗的临床反应作为金标准评估 IS6110-TaqMan 检测时，IS6110-TaqMan 检测的敏感性为 87.9%，特异性为 98%。还评估了 IS6110-TaqMan 检测与痰涂片显微镜检查的性能，敏感性为 79.7%，特异性为 94.8%。
IS6110-TaqMan 对肺结核的诊断快速、敏感、特异。
当与常规实验室检查、患者的临床和流行病学标准结合使用时，IS6110-TaqMan 检测是一种有前途的肺结核辅助诊断工具，从而提高了诊断的敏感性和特异性。
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