State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Dayangfang 8, Anwai Beiyuan, Beijing 100012, China.
J Hazard Mater. 2013 Feb 15;246-247:34-43. doi: 10.1016/j.jhazmat.2012.12.010. Epub 2012 Dec 12.
Berberine is a broad-spectrum antibiotic extensively used in personal medication. The production of berberine results in the generation of wastewater containing concentrated residual berberine. However, few related studies up to date focus on berberine removal from wastewaters. In this study, a lab-scale upflow anaerobic sludge blanket (UASB)-membrane bioreactor (MBR) process was developed for berberine removal from synthetic wastewater. The performance of the UASB-MBR system on berberine, COD and NH(4)(+)--N removal was investigated at different berberine loadings. And the effects of berberine on bacterial communities were evaluated using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Results showed that, as the increase of berberine loadings, UASB performance was affected remarkably, whereas, efficient and stable performance of MBR ensured the overall removal rates of berberine, COD and NH(4)(+)--N consistently reached up to 99%, 98% and 98%, respectively. Significant shifts of bacterial community structures were detected in both UASB and MBR, especially in the initial operations. Along with the increase of berberine loadings, high antibiotic resisting species and some functional species, i.e. Acinetobacter sp., Clostridium sp., Propionibacterium sp., and Sphingomonas sp. in UASB, as well as Sphingomonas sp., Methylocystis sp., Hydrogenophaga sp. and Flavobacterium sp. in MBR were enriched in succession.
小檗碱是一种广泛应用于个人医疗的广谱抗生素。小檗碱的生产会产生含有浓缩残留小檗碱的废水。然而，迄今为止，很少有相关研究关注从废水中去除小檗碱。在这项研究中，开发了一种实验室规模的上流式厌氧污泥床（UASB）-膜生物反应器（MBR）工艺，用于从合成废水中去除小檗碱。考察了 UASB-MBR 系统在不同小檗碱负荷下对小檗碱、COD 和 NH(4)(+)--N 的去除性能。并采用聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）评价了小檗碱对细菌群落的影响。结果表明，随着小檗碱负荷的增加，UASB 的性能受到显著影响，而 MBR 的高效稳定性能确保了小檗碱、COD 和 NH(4)(+)--N 的总去除率分别稳定达到 99%、98%和 98%。在 UASB 和 MBR 中均检测到细菌群落结构的显著变化，尤其是在初始运行阶段。随着小檗碱负荷的增加，高抗生素抗性物种和一些功能物种在 UASB 中相继富集，如不动杆菌属、梭菌属、丙酸杆菌属和鞘氨醇单胞菌属，而在 MBR 中则为鞘氨醇单胞菌属、甲基球菌属、噬氢菌属和黄杆菌属。
