Wang Lamei, Li Shili, Gou Kemian, Luo Yuzhu
College Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
Sheng Wu Gong Cheng Xue Bao. 2012 Sep;28(9):1106-17.
The possibility of heterologous expression of human Stearoyl-CoA Desaturase (scd1) was investigated. The scd1 encoding sequence was inserted into the pNZ8149 to generate the pNZ8149-scd1 expression plasmids. Then we introduced the pNZ8149-scd1 construct into the Lactococcus lactis NZ3900 to investigate its enzyme activity. The results show that heterologous expressed SCD1 enzyme resulted in a 92%-169% increase in the C16:1n-7 and a 53-127% increase in the C18:1n-7 (P<0.05). The SCD1 enzyme was capable of producing n-7 fatty acids in Lactococcus lactis efficiently. It also suggests that the fatty acid desaturases can be heterologous expressed in Lactococcus lactis to produce the helpful fatty acids.
研究了人硬脂酰辅酶A去饱和酶（scd1）异源表达的可能性。将scd1编码序列插入pNZ8149中以产生pNZ8149-scd1表达质粒。然后我们将pNZ8149-scd1构建体导入乳酸乳球菌NZ3900中以研究其酶活性。结果表明，异源表达的SCD1酶使C16:1n-7增加了92%-169%，C18:1n-7增加了53%-127%（P<0.05）。SCD1酶能够在乳酸乳球菌中高效产生n-7脂肪酸。这也表明脂肪酸去饱和酶可以在乳酸乳球菌中异源表达以产生有益的脂肪酸。
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