[多肽P110增强顺铂对结肠癌HCT-116细胞及小鼠异种移植瘤的生长抑制作用]

[Polypeptide P110 enhances the growth-inhibitory effect of cisplatin on colon cancer HCT-116 cells and xenotransplanted tumors in mice].

作者信息

Chen Zhi-qiang, Cui Wei, Tan Shi-yun, Chen Jian-hua, Liu Jun, Zhang Jian, Chen Cai-hong

机构信息

Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2012 Nov;34(11):816-20. doi: 10.3760/cma.j.issn.0253-3766.2012.11.004.

DOI:10.3760/cma.j.issn.0253-3766.2012.11.004

PMID:23291128

Abstract

OBJECTIVE

To observe the growth-inhibitory effect of polypeptide P110, designed with G3BP protein targets, plus cisplatin on human colon cancer HCT-116 cells and mouse colon cancer C26 xenotransplanted tumors in mice.

METHODS

The proliferation inhibition of HCT-116 cells and HUVEC cells in vitro was evaluated by MTT assay. A mouse model of xenotransplanted C26 mouse colon cancer was established. The inhibitory effects of P110 and cisplatin at different concentrations on C26 xenotransplanted tumors were assessed.

RESULTS

P110 enhanced the inhibitory effect of cisplatin on proliferation of HCT-116 cells. By treated with 20 µmol/LP110 + 10, 30, 90 µmol/L cisplatin, the proliferation inhibitory rates were (43.3 ± 3.2)%, (46.4 ± 4.6)% and (47.6 ± 5.8)%, respectively, significantly higher than that in the cisplatin group (P < 0.05). 20 µmol/L P110 + 10 µmol/L cisplatin effectively killed HCT-116 cells, whereas with less toxicity to HUVEC cells. The tumor inhibition rates in mice of P110 (25 mg/kg, 50 mg/kg, 100 mg/kg) plus cisplatin (1 mg/kg) were 23.0%, 30.4% and 34.2%, respectively. While, the tumor inhibition rates in mice of the cisplatin group (1 mg/kg) was 22.7%. Compared with cisplatin at the same concentration, the tumor inhibition rates in mice of the P110 plus cisplatin groups were all increased.

CONCLUSIONS

P110 can enhance the growth inhibitory effects of cisplatin on HCT-116 cells and C26 xenotransplanted tumors in mice. P110 plus cisplatin can reduce the effective dose of cisplatin and decrease the toxicity of cisplatin.

摘要

目的

观察以G3BP蛋白为靶点设计的多肽P110联合顺铂对人结肠癌HCT-116细胞及小鼠结肠癌C26移植瘤的生长抑制作用。

方法

采用MTT法检测HCT-116细胞和人脐静脉内皮细胞（HUVEC）的体外增殖抑制情况。建立C26小鼠结肠癌移植瘤模型，评估不同浓度的P110和顺铂对C26移植瘤的抑制作用。

结果

P110增强了顺铂对HCT-116细胞增殖的抑制作用。用20 μmol/L P110 + 10、30、90 μmol/L顺铂处理后，增殖抑制率分别为(43.3 ± 3.2)%、(46.4 ± 4.6)%和(47.6 ± 5.8)%，显著高于顺铂组（P < 0.05）。20 μmol/L P110 + 10 μmol/L顺铂能有效杀伤HCT-116细胞，而对HUVEC细胞毒性较小。P110（25 mg/kg、50 mg/kg、100 mg/kg）联合顺铂（1 mg/kg）对小鼠肿瘤的抑制率分别为23.0%、30.4%和34.2%。而顺铂组（1 mg/kg）小鼠的肿瘤抑制率为22.7%。与相同浓度的顺铂相比，P110联合顺铂组小鼠的肿瘤抑制率均有所提高。