促红细胞生成素缺乏的小鼠中，红细胞变形性略有降低。

Red blood cell deformability is very slightly decreased in erythropoietin deficient mice.

作者信息

Pichon Aurélien, Lamarre Yann, Voituron Nicolas, Marchant Dominique, Vilar José, Richalet Jean-Paul, Connes Philippe

机构信息

Université Paris 13, Sorbonne Paris Cité, Laboratoire «Réponses cellulaires et fonctionnelles à l'hypoxie» EA2363, Bobigny, France.

UMR Inserm S 665, Université des Antilles et de la Guyane/Université Paris Diderot, Hôpital Ricou, CHU de Pointe-à-Pitre, Pointe-à-Pitre, Guadeloupe.

出版信息

Clin Hemorheol Microcirc. 2014;56(1):41-6. doi: 10.3233/CH-121654.

DOI:10.3233/CH-121654

PMID:23302595

Abstract

The present study compared the hemorheological properties between Epo-TAgh mice (a model of erythropoietin deficient mice) and wild-type (WT) control mice. Blood viscosity was determined at several shear rates using a cone-plate viscometer at native and adjusted hematocrit (i.e. 40%). Red blood cell (RBC) deformability was measured by ecktacytometry at several shear stresses and RBC aggregation properties by backscattered technique at adjusted hematocrit (i.e. 40%). Epo-TAgh mice had severe anemia (very low hematocrit), decreased blood viscosity at native hematocrit and slightly reduced RBC deformability at high shear stresses in comparison with WT mice. Blood viscosity at adjusted hematocrit (i.e. 40%) was not different between WT and Epo-TAgh mice. RBC aggregation did not differ between the two mice models. These findings suggest a role of erythropoietin in the regulation of RBC deformability.

摘要

本研究比较了促红细胞生成素缺陷小鼠（Epo-TAgh小鼠）与野生型（WT）对照小鼠的血液流变学特性。使用锥板式粘度计在自然血细胞比容和调整后的血细胞比容（即40%）下的多个剪切速率下测定血液粘度。通过红细胞变形性测定法在多个剪切应力下测量红细胞（RBC）变形性，并通过背散射技术在调整后的血细胞比容（即40%）下测量RBC聚集特性。与WT小鼠相比，Epo-TAgh小鼠患有严重贫血（血细胞比容极低），在自然血细胞比容下血液粘度降低，在高剪切应力下RBC变形性略有降低。WT小鼠和Epo-TAgh小鼠在调整后的血细胞比容（即40%）下的血液粘度没有差异。两种小鼠模型的RBC聚集没有差异。这些发现表明促红细胞生成素在调节RBC变形性中起作用。